Gentamicin activates rat mesangial cells. A role for platelet activating factor

Gentamicin activates cultured rat mesangial cells. A role for platelet activating factor. Gentamicin-induced decreases in glomerular filtration rate have been associated with a marked decline in the glomerular capillary ultrafiltration coefficient which could be mediated by mesangial cell contractio...

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Bibliographic Details
Published in:Kidney international 1995-05, Vol.47 (5), p.1346-1353
Main Authors: Rodriguez-Barbero, Alicia, Rodriguez-Lopez, Ana M., Gonzalez-Sarmiento, Rogelio, López-Novoa, Jose M.
Format: Article
Language:English
Subjects:
Alprazolam - pharmacology
Animals
Base Sequence
Biological and medical sciences
Calcium - metabolism
Cell Division - drug effects
Cells, Cultured
Diterpenes
Drug toxicity and drugs side effects treatment
Genes, fos - drug effects
Gentamicins - pharmacology
Ginkgolides
Glomerular Filtration Rate - drug effects
Glomerular Mesangium - drug effects
Glomerular Mesangium - metabolism
Glomerular Mesangium - pathology
Lactones - pharmacology
Leucine - analogs & derivatives
Leucine - pharmacology
Medical sciences
Molecular Sequence Data
Pharmacology. Drug treatments
Platelet Activating Factor - antagonists & inhibitors
Platelet Activating Factor - physiology
Rats
Rats, Wistar
RNA, Messenger - drug effects
Toxicity: urogenital system
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Summary:Gentamicin activates cultured rat mesangial cells. A role for platelet activating factor. Gentamicin-induced decreases in glomerular filtration rate have been associated with a marked decline in the glomerular capillary ultrafiltration coefficient which could be mediated by mesangial cell contraction or release of vasoactive hormones. We studied the effect of gentamicin on mesangial cells proliferation, contraction and Ca2+ mobilization. Moreover, we attempted to assess a possible role of platelet activating factor (PAF) as a mediator of the observed effects of gentamicin on mesangial cells. Gentamicin induced a reduction of planar surface area of cultured rat mesangial cells that was blunted by the PAF-antagonist, BN-52021. Gentamicin induced an increase in [Ca2+]i that was inhibited by BN-52021. Gentamicin also stimulated [3H]thymidine incorporation into DNA, an effect that was also reduced by BN-52021, and by other two structurally different PAF receptor antagonists: alprazolam and BB-823. Gentamicin induced c-fos mRNA expression in quiescent mesangial cells. Gentamicin stimulated the synthesis and release of PAF in cultured rat mesangial cells. The present studies demonstrate that gentamicin activates mesangial cell function. These actions seem to be mediated, at least in part, by PAF synthesis and release.
ISSN:0085-2538
1523-1755
DOI:10.1038/ki.1995.190