Specific T cell recognition of minimally homologous peptides: Evidence for multiple endogenous Ligands

The T cell receptor (TCR) can interact with a spectrum of peptides as part of its ligand, Including the immunogenic peptide, variants of this peptide, and apparently unrelated peptides. The basis of this broad specificity for ligand was investigated by substitution analysis of a peptide antigen and...

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Bibliographic Details
Published in:Immunity (Cambridge, Mass.) Mass.), 1995-06, Vol.2 (6), p.655-663
Main Authors: Evavold, Brian D., Sloan-Lancastert, Joanne, Wilson, K.Jeff, Rothbard, Jonathan B., Allen, Paul M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Apoptosis - immunology
B-Lymphocytes - immunology
Cell Line
Epitopes - immunology
Ligands
Lymphocyte Activation
Mice
Molecular Sequence Data
Peptides - immunology
Receptors, Antigen, T-Cell - agonists
Receptors, Antigen, T-Cell - antagonists & inhibitors
Receptors, Antigen, T-Cell - immunology
T-Lymphocytes - immunology
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Summary:The T cell receptor (TCR) can interact with a spectrum of peptides as part of its ligand, Including the immunogenic peptide, variants of this peptide, and apparently unrelated peptides. The basis of this broad specificity for ligand was investigated by substitution analysis of a peptide antigen and functional testing using a B cell apoptosls assay. A peptide containing as few as 1 aa in common with this peptide could stimulate a specific T cell response. Two endogenous ligands, an agonist and a partial agonist, were readily Identified from a search of the SwissProt database, indicating that multiple endogenous ligands likely exist for a given T cell. These findings strongly support the concept that one TCR has the ability to interact productively with multiple different Iigands, and provide evidence that such ligands exist in the endogenous peptide repertoire.
ISSN:1074-7613
1097-4180
DOI:10.1016/1074-7613(95)90010-1