Gonadotrophin surge-attenuating factor bioactivity is present in follicular fluid from naturally cycling women

Rat pituitary monolayer bioassays were used to compare gonadotrophin surge-attenuating factor (GnSAF) bioactivity in follicular fluid from 12 follicles in 10 spontaneously cycling women with that in pooled follicular fluid from women undergoing ovulation induction. Expressed as ED50s (μl follicular...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1995-01, Vol.10 (1), p.68-74
Main Authors: Fowler, P.A., Fahy, U., Culler, M.D., Knight, P.G., Wardle, P.G., McLaughlin, E.A., Cunningham, P., Fraser, M., Hull, M.G.R., Templeton, A.
Format: Article
Language:English
Subjects:
Adult
Animals
Biological and medical sciences
Biological Assay - methods
Cells, Cultured
Estradiol - administration & dosage
Feedback
Female
Fertilization in Vitro
Follicle Stimulating Hormone - metabolism
follicular fluid
Follicular Fluid - metabolism
Fundamental and applied biological sciences. Psychology
GnRH
GnSAF
Gonadal Hormones
Gonadal Steroid Hormones - analysis
Gonadal Steroid Hormones - metabolism
Hormone metabolism and regulation
Humans
Inhibins - metabolism
Luteinizing Hormone - metabolism
Mammalian female genital system
Menstrual Cycle - metabolism
Ovulation Induction
Pituitary Gland
Progesterone - administration & dosage
Proteins - analysis
Proteins - metabolism
Rats
spontaneous cycles
Vertebrates: reproduction
women
Citations: Items that cite this one
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Summary:Rat pituitary monolayer bioassays were used to compare gonadotrophin surge-attenuating factor (GnSAF) bioactivity in follicular fluid from 12 follicles in 10 spontaneously cycling women with that in pooled follicular fluid from women undergoing ovulation induction. Expressed as ED50s (μl follicular fluid/well producing 50% of maximal effect), GnSAF bioactivity was detectable in all spontaneous follicular fluid samples (1.4–33.3 μl/well) and in follicular fluid from women undergoing ovulation induction (6.8 μl/well). This GnSAF bioactivity was unaffected by pre-incubation with an inhibin antibody. When the data were grouped according to whether the recovered oocytes fertilized in vitro or not, the fertilized group contained significantly greater GnSAF bioactivity than the unfertilized group (5.3 ± 1.1 and 14.1 ± 2.6 μl/well respectively, P < 0.05). While both inhibin bioactivity (9.7 ± 1.4 and 28.9 ± 12.1 μl/well) and immunoreactivity (36.8 ± 2.2 and 21.0 ± 3.0 and ng/ml) were also greater (P < 0.01) in the fertilized compared with the unfertilized groups respectively, there were no other significant differences between the two groups. We conclude that GnSAF is found in follicular fluid from spontaneously cycling women, supporting in-vivo evidence for the involvement of GnSAF in feedback control of the ovary-pituitary axis.
ISSN:0268-1161
1460-2350
DOI:10.1093/humrep/10.1.68