Structure of the Cell Wall Anchor of Surface Proteins in Staphylococcus aureus

Many surface proteins are anchored to the cell wall of Gram-positive bacteria and are involved in the pathogenesis of these organisms. A hybrid molecule was designed that, when expressed in Staphylococcus aureus, was anchored to the cell wall and could be released by controlled enzymatic digestion....

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1995-04, Vol.268 (5207), p.103-106
Main Authors: Schneewind, Olaf, Fowler, Audree, Faull, Kym F.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Average linear density
Bacteria
Bacterial cell walls
Bacterial Proteins - chemistry
Bacteriology
Base Sequence
Biogenesis of cell structures, supramolecular organization
Biological and medical sciences
Carrier Proteins - chemistry
Cell Wall - chemistry
Cell walls
Cellular biology
Chromatography, Affinity
Chromatography, High Pressure Liquid - methods
Electrophoresis, Polyacrylamide Gel
Fractions
Fundamental and applied biological sciences. Psychology
glycine
Gram positive bacteria
Hybridity
Leaders
Literary Devices
Males
Maltose-Binding Proteins
Mass spectroscopy
Membrane proteins
Membrane Proteins - chemistry
Microbiology
Molecular Sequence Data
Molecules
Observation
Physiological aspects
Proteins
Recombinant Fusion Proteins - chemistry
Staphylococcal Protein A - chemistry
Staphylococcus
Staphylococcus aureus
Staphylococcus aureus - chemistry
Streptococcus
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Summary:Many surface proteins are anchored to the cell wall of Gram-positive bacteria and are involved in the pathogenesis of these organisms. A hybrid molecule was designed that, when expressed in Staphylococcus aureus, was anchored to the cell wall and could be released by controlled enzymatic digestion. By a combination of molecular biology and mass spectrometry techniques, the structure of the cell wall anchor of surface proteins in S. aureus was revealed. After cleavage of surface proteins between threonine and glycine of the conserved LPXTG motif, the carboxyl of threonine is amide-linked to the free amino group of the pentaglycine crossbridge in the staphylococcal cell wall.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.7701329