Cytokine secretion and eicosanoid production in the peripheral blood mononuclear cells of MS patients undergoing dietary supplementation with n-3 polyunsaturated fatty acids

To demonstrate the influence of n-3 PUFA supplementation on cytokine and eicosanoid production in peripheral blood mononuclear cells (PBMCs) of MS patients (MSP), we investigated the impact of a 6-month dietary supplementation with these fatty acids on the levels of interleukin-lbeta (IL-1β), IL-2,...

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Bibliographic Details
Published in:Journal of neuroimmunology 1995-02, Vol.56 (2), p.143-153
Main Authors: Gallai, Virgilio, Sarchielli, Paola, Trequattrini, Alberto, Franceschini, Maristella, Floridi, Ardesio, Firenze, Caterina, Alberti, Andrea, Benedetto, Daniela Di, Stragliotto, Eduardo
Format: Article
Language:English
Subjects:
Adult
Cytokines
Cytokines - biosynthesis
Dinoprostone - biosynthesis
Fatty Acids, Omega-3 - administration & dosage
Humans
Interferon-gamma - biosynthesis
Interleukin-1 - biosynthesis
Interleukin-2 - biosynthesis
Leukocytes, Mononuclear - metabolism
Leukotriene B4
Leukotriene B4 - biosynthesis
Lipopolysaccharides - pharmacology
Middle Aged
Mononuclear cells
Multiple sclerosis
Multiple Sclerosis - metabolism
n-3 PUFA supplementation
Prostaglandin E2
Receptors, Interleukin-2 - analysis
Soluble interleukin-2 receptors
Tumor Necrosis Factor-alpha - biosynthesis
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Summary:To demonstrate the influence of n-3 PUFA supplementation on cytokine and eicosanoid production in peripheral blood mononuclear cells (PBMCs) of MS patients (MSP), we investigated the impact of a 6-month dietary supplementation with these fatty acids on the levels of interleukin-lbeta (IL-1β), IL-2, interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) in the supernatants of stimulated PBMCs and serum soluble IL-2 receptors in a group of 20 relapsing-remitting (R-R) MSP and a group of 15 age-matched control individuals (CI). The production of PGE2 and LTB4 in the stimulated PBMCs was also assessed in patient and control groups supplemented with n-3 PUFAs. In both groups, n-3 PUFA supplementation led to a significant decrease in the levels of IL-1β and TNF-α, and this reduction was more pronounced in the 3rd and 6th month of supplementation. An analogous decrease was observed in the levels of IL-2 and IFN-γ produced by stimulated PBMCs, and in the levels of serum soluble IL-2 receptors, n-3 PUFA supplementation also appeared to significantly affect prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) production in PBMCs, both in MSP and the control group. The reduced production of these proinflammatory eicosanoids, and the decrease of some cytokines with an immunohenancing effect as a consequence of n-3 PUFA supplementation, could modulate some immune functions which have been demonstrated to be altered in MSP.
ISSN:0165-5728
1872-8421
DOI:10.1016/0165-5728(94)00140-J