Catalytic heterogeneity of polyclonal DNA-hydrolyzing antibodies from the sera of patients with multiple sclerosis

Various catalytic antibodies or abzymes have been detected recently in the sera of patients with several autoimmune pathologies, where their presence is most probably associated with autoimmunization. Recently we have shown that DNase activity is associated with IgGs from the sera of patients with m...

Full description

Saved in:
Bibliographic Details
Published in:Immunology letters 2001-04, Vol.76 (3), p.163-167
Main Authors: Baranovskii, Andrey G., Ershova, Nadezhda A., Buneva, Valentina N., Kanyshkova, Tat'yana G., Mogelnitskii, Alexander S., Doronin, Boris M., Boiko, Alexey N., Gusev, Evgenii I., Favorova, Olga O., Nevinsky, Georgy A.
Format: Article
Language:English
Subjects:
Antibodies, Catalytic - isolation & purification
Antibodies, Catalytic - metabolism
Catalysis
Catalytic IgG
DNA - metabolism
DNase activity
Exodeoxyribonucleases - isolation & purification
Exodeoxyribonucleases - metabolism
Exonucleases - metabolism
Human blood
Humans
Hydrogen-Ion Concentration
Immunoglobulin Fab Fragments - isolation & purification
Immunoglobulin Fab Fragments - metabolism
Immunoglobulin G - isolation & purification
Immunoglobulin G - metabolism
Kinetics
Multiple sclerosis
Multiple Sclerosis - immunology
Oligodeoxyribonucleotides - metabolism
Phosphodiesterase I
Phosphoric Diester Hydrolases - metabolism
Substrate Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Various catalytic antibodies or abzymes have been detected recently in the sera of patients with several autoimmune pathologies, where their presence is most probably associated with autoimmunization. Recently we have shown that DNase activity is associated with IgGs from the sera of patients with multiple sclerosis (MS) but not with those from the sera of normal humans. Here we present evidence showing that MS IgG, its F(ab) fragments, and separated L-chains catalyze DNA hydrolysis. The properties of the DNase activity of these polyclonal IgGs distinguish them from other known human DNases. In addition, their specific activities with different oligonucleotide substrates and the range of optimal pHs, apparent K M values and substrate specificities varied widely for different patients. The findings speak in favor of the generation by the immune systems of individual patients of a variety of polyclonal catalytic IgG pools, from relatively small to extremely large ones.
ISSN:0165-2478
1879-0542
DOI:10.1016/S0165-2478(01)00185-7