Plasmid transfer and susceptibility to antibiotics in the halophilic phototrophs Rhodovibrio salinarum and Rhodothalassium salexigens

Abstract The present study defines a series of genetic procedures to be used for molecular studies in photosynthetic halophilic species such as Rhodovibrio salinarum and Rhodothalassium salexigens. In both species, the minimal inhibitory concentrations for the antibiotics tetracycline, rifampicin, c...

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Bibliographic Details
Published in:FEMS microbiology letters 2001-04, Vol.197 (1), p.117-121
Main Authors: Borghese, Roberto, Zagnoli, Alessandra, Zannoni, Davide
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotic
Antibiotics
Bacteria - drug effects
Bacteria - genetics
Bacteria - growth & development
Bacteriology
Biological and medical sciences
Biotechnology
Chloramphenicol
Chloromycetin
Conjugation
Conjugation, Genetic
Dependence
E coli
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Genetics
Growth rate
Halophiles
Halophilic phototroph
Kanamycin
Methods. Procedures. Technologies
Microbial Sensitivity Tests
Microbiology
Photosynthesis
Plasmids
Plasmids - genetics
Rhodothalassium salexigens
Rhodovibrio salinarum
Rifampin
Sodium chloride
Sodium Chloride - metabolism
Species
Spectinomycin
Streptomycin
Sulfur
Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Summary:Abstract The present study defines a series of genetic procedures to be used for molecular studies in photosynthetic halophilic species such as Rhodovibrio salinarum and Rhodothalassium salexigens. In both species, the minimal inhibitory concentrations for the antibiotics tetracycline, rifampicin, chloramphenicol, spectinomycin, streptomycin, and kanamycin were determined. In addition, conjugal transfer of IncP and IncQ plasmids from Escherichia coli was demonstrated and the resistance markers expressed in these halophiles were determined. Finally, Rth. salexigens growth dependence on variable salt concentrations was measured: maximal growth rates were seen at 6% and 4% NaCl under phototrophic and chemotrophic conditions, respectively. To the best of our knowledge, this is the first report analyzing the genetic properties of two representative species of halophilic purple non-sulfur phototrophs.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2001.tb10592.x