[PSI+] prion generation in yeast: characterization of the ‘strain’ difference

The yeast cytoplasmically‐inherited nonsense suppressor [PSI+] determinant is presumed to be a manifestation of the aggregated prion‐like state of the Sup35 protein. Overexpression of the Sup35 protein induces generation of [PSI+] determinants with various suppressor efficiency and mitotic stabiliti...

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Bibliographic Details
Published in:Yeast (Chichester, England) England), 2001-04, Vol.18 (6), p.489-497
Main Authors: Kochneva‐Pervukhova, Natalia V., Chechenova, Maria B., Valouev, Igor A., Kushnirov, Vitaly V., Smirnov, Vladimir N., Ter‐Avanesyan, Michael D.
Format: Article
Language:English
Subjects:
Alleles
Blotting, Western
Cell Aggregation - genetics
Cell Cycle
eRF3
Fungal Proteins - biosynthesis
Fungal Proteins - genetics
Gene Expression Regulation, Fungal - genetics
nonsense suppressors
Peptide Termination Factors
Phenotype
Plasmids
prion strains
Prions - genetics
Prions - metabolism
PSI
q@u+ prion protein
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Species Specificity
Sup35 protein
Sup35p
Suppression, Genetic - genetics
yeast
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Summary:The yeast cytoplasmically‐inherited nonsense suppressor [PSI+] determinant is presumed to be a manifestation of the aggregated prion‐like state of the Sup35 protein. Overexpression of the Sup35 protein induces generation of [PSI+] determinants with various suppressor efficiency and mitotic stabilities. Here, we demonstrate that the relative frequency of appearance of [PSI+] with different properties depends on the SUP35 allele used to induce their generation. The difference in properties of [PSI+] determinants was preserved after their transmission from one yeast strain to another. This difference correlated with variation in properties of the Sup35 protein. A novel type of prion instability was observed: some [PSI+] with weak suppressor efficiency could convert spontaneously into strong suppressor determinants. Copyright © 2001 John Wiley & Sons, Ltd.
ISSN:0749-503X
1097-0061
DOI:10.1002/yea.700