Latex based, rapid and easy assay for human leptospirosis in a single test format

Leptospirosis is an often severe disease which requires prompt treatment. Laboratory testing is required to reach a valid diagnosis. An agglutination assay for the detection of Leptospira‐specific antibodies consisting of individually wrapped agglutination cards containing a stable, dried detection...

Full description

Saved in:
Bibliographic Details
Published in:Tropical medicine & international health 2001-02, Vol.6 (2), p.114-118
Main Authors: Smits, Henk L., Chee, Howard D., Eapen, C. K., Kuriakose, Mariamma, Sugathan, Sheela, Gasem, M. Hussein, Yersin, Claude, Sakasi, David, Lai‐a‐Fat, Rudy F. M., Hartskeerl, Rudy A., Liesdek, Bernadette, Abdoel, Theresia H., Goris, Marga G. A., Gussenhoven, George C.
Format: Article
Language:English
Subjects:
Antibodies, Bacterial - blood
Antigens, Bacterial
Bacterial diseases
Biological and medical sciences
ELISA
Enzyme-Linked Immunosorbent Assay
Human bacterial diseases
Humans
Immunoglobulin M - blood
Infectious diseases
latex agglutination assay
Latex Fixation Tests - methods
Leptospira - immunology
leptospirosis
Leptospirosis - blood
Leptospirosis - diagnosis
Leptospirosis - immunology
Medical sciences
microscopic agglutination assay
Miscellaneous
Sensitivity and Specificity
serodiagnosis
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Leptospirosis is an often severe disease which requires prompt treatment. Laboratory testing is required to reach a valid diagnosis. An agglutination assay for the detection of Leptospira‐specific antibodies consisting of individually wrapped agglutination cards containing a stable, dried detection reagent is evaluated. The assay is simply performed by suspending the dried reagent with a drop of serum. The result is obtained within 30 s. The sensitivity of the assay varied with the stage of the disease and was 72.3% for samples collected during the first 10 days of the illness and 88.2% for samples collected at a later stage. The specificity was 93.9% and 89.8%, respectively. These characteristics make the test ideal for use in areas where the disease is common and where laboratory support is not routinely available.
ISSN:1360-2276
1365-3156
DOI:10.1046/j.1365-3156.2001.00675.x