Diagnosing visceral leishmaniasis with the recombinant K39 strip test: experience from the Sudan

We compared a strip test employing recombinant K39 (rK39) antigen and protein A/colloidal gold as read‐out agents with the rK39 ELISA for IgM and IgG antibodies and the direct agglutination test (DAT) using 55 sera from patients with parasitologically confirmed visceral leishmaniasis (VL). The rK39...

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Bibliographic Details
Published in:Tropical medicine & international health 2001-02, Vol.6 (2), p.108-113
Main Authors: Zijlstra, E. E., Nur, Y., Desjeux, P., Khalil, E. A. G., El‐Hassan, A. M., Groen, J.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Agglutination Tests
Animals
Antibodies, Protozoan - blood
Antibodies, Protozoan - immunology
Antigens, Protozoan - immunology
Biological and medical sciences
Bone Marrow - parasitology
Child
Child, Preschool
diagnosis
Enzyme-Linked Immunosorbent Assay
Female
Human protozoal diseases
Humans
Immunoglobulin G - blood
Immunoglobulin M - blood
Infectious diseases
Leishmania donovani - immunology
Leishmania donovani - isolation & purification
Leishmaniasis, Visceral - diagnosis
Leishmaniasis, Visceral - immunology
Leshmaniasis
Lymph Nodes - parasitology
Male
Medical sciences
Middle Aged
Parasitic diseases
Protozoal diseases
Protozoan Proteins - immunology
Reagent Strips
Recombinant Proteins - immunology
rK39 strip test
Sensitivity and Specificity
Spleen - parasitology
Sudan
Time Factors
Tropical medicine
visceral leishmaniasis
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Summary:We compared a strip test employing recombinant K39 (rK39) antigen and protein A/colloidal gold as read‐out agents with the rK39 ELISA for IgM and IgG antibodies and the direct agglutination test (DAT) using 55 sera from patients with parasitologically confirmed visceral leishmaniasis (VL). The rK39 strip test was positive in 37/55 (67%), the DAT in 50/55 (91%) at ≥ 1 : 1600 cut‐off value and in 47/55 (85%) at ≥ 1 : 6400 cut‐off value. The rK39‐ELISA gave positive IgG results for all sera; those who had a positive strip test had significantly higher IgG levels than those with a negative strip test (31.1 (SD=3.6) and 17.7 U/ml (SD=9.8), respectively, P 
ISSN:1360-2276
1365-3156
DOI:10.1046/j.1365-3156.2001.00680.x