Molecular cloning of the chicken progesterone receptor

To define the functional domains of the progesterone receptor required for gene regulation, complementary DNA (cDNA) clones encoding the chicken progesterone receptor have been isolated from a chicken oviduct $\lambda $gt11 cDNA expression library. Positive clones expressed antigenic determinants th...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1986-08, Vol.233 (4765), p.767-770
Main Authors: Conneely, O.M, Sullivan, W.P, Toft, D.O, Birnbaumer, M, Cook, R.G, Maxwell, B.L, Zarucki-Schulz, T, Greene, G.L, Schrader, W.T, O'Malley, B.W
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
Amino acids
Animals
Antibodies
Antibodies, Monoclonal
Base Sequence
Biological and medical sciences
Biotechnology
Central nervous system
CHICKENS
CLONACION
CLONAGE
CLONING
Cloning, Molecular
Complementary DNA
Cross Reactions
DNA
DNA - metabolism
Epitopes - analysis
Female
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genetic aspects
Genetic engineering
Genetic research
Genetic technics
GENETICA
GENETICS
GENETIQUE
Hormones
Humans
Methods. Procedures. Technologies
Nucleic Acid Hybridization
Oviducts
Oviducts - metabolism
POLLO
POULET
PROGESTERONA
PROGESTERONE
Progesterone receptors
Proteins
Receptors
Receptors, Progesterone - genetics
Reoviridae
RNA, Messenger - genetics
Species Specificity
Spinal cord
Viruses
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Summary:To define the functional domains of the progesterone receptor required for gene regulation, complementary DNA (cDNA) clones encoding the chicken progesterone receptor have been isolated from a chicken oviduct $\lambda $gt11 cDNA expression library. Positive clones expressed antigenic determinants that cross-reacted with six monospecific antibodies derived from two independent sources. A 36-amino acid peptide sequence obtained by microsequencing of purified progesterone receptor was encoded by nucleotide sequences in the longer cDNA clone. Analysis of the amino acid sequence of the progesterone receptor deduced from the cDNA clones revealed a cysteine-rich region that was homologous to a region found in the estrogen and glucocorticoid receptors and to the avian erythroblastosis virus gag-erb-A fusion protein. Northern blot analysis with chicken progesterone receptor cDNA's indicated the existence of at least three messenger RNA species. These messages were found only in oviduct and could be induced by estrogens.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2426779