Cutting Edge: Nociceptin Stimulates Neutrophil Chemotaxis and Recruitment: Inhibition by Aspirin-Triggered-15-Epi-Lipoxin A4

The nociceptin receptor (Noci-R) is a G protein-coupled receptor present in neural tissues and its activation by nociceptin is involved in the processing of pain signals. Here, we report that Noci-R is present and functional on peripheral blood polymorphonuclear leukocytes (PMN). Human PMN express m...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2001-03, Vol.166 (6), p.3650-3654
Main Authors: Serhan, Charles N, Fierro, Iolanda M, Chiang, Nan, Pouliot, Marc
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents, Non-Steroidal - pharmacology
Aspirin - pharmacology
Calcium Signaling
Cell Movement - physiology
Chemotactic Factors - antagonists & inhibitors
Chemotactic Factors - physiology
Chemotaxis, Leukocyte - drug effects
Humans
Hydroxyeicosatetraenoic Acids - physiology
Injections, Intradermal
Intracellular Fluid - physiology
Lipoxins
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Neutrophil Infiltration - drug effects
Neutrophils - drug effects
Neutrophils - metabolism
Neutrophils - physiology
Nociceptin
Nociceptin Receptor
Opioid Peptides - administration & dosage
Opioid Peptides - antagonists & inhibitors
Opioid Peptides - metabolism
Opioid Peptides - physiology
Receptors, Opioid - biosynthesis
Receptors, Opioid - genetics
RNA, Messenger - biosynthesis
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Summary:The nociceptin receptor (Noci-R) is a G protein-coupled receptor present in neural tissues and its activation by nociceptin is involved in the processing of pain signals. Here, we report that Noci-R is present and functional on peripheral blood polymorphonuclear leukocytes (PMN). Human PMN express mRNA for Noci-R, its nucleotide sequence determined, and specific binding with [(125)I]-labeled nociceptin gave an apparent K(d) approximately 1.5 nM for this PMN opioid receptor. Nociceptin evoked PMN chemotaxis with maximal activity at 100 pM, without intracellular Ca(2+) mobilization. When injected in murine air pouches, nociceptin elicited leukocyte infiltration in a concentration-dependent fashion. Nociceptin-stimulated PMN infiltration was inhibited by treating mice with a synthetic analog of the aspirin-triggered lipid mediator 15-epi-lipoxin A(4). The present results identify nociceptin as a potent chemoattractant and provide a novel link between the neural and immune systems that are blocked by aspirin-triggered lipid mediators and may be relevant in neurogenic inflammation.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.166.6.3650