Expression and Down-Regulation by Retinoic Acid of IGF Binding Protein-2 and -4 in Medium from Human Neuroblastoma Cells

Insulin‐like growth factors (IGFs) regulate the autocrine/paracrine growth of neuroblastomas. The IGFs bind to specific binding proteins (IGFBPs) which modulate their biological activity. We investigated, by Western ligand blotting (WLB), the presence of IGFBPs and their possible modulation by retin...

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Bibliographic Details
Published in:Journal of neuroendocrinology 1994-08, Vol.6 (4), p.409-413
Main Authors: Bernardini, Sergio, Cianfarani, Stefano, Spagnoli, Anna, Annicchiarico-Petruzzelli, Margherita, Melino, Gerry, Massoud, Renato, Boscherini, Brunetto, Finazzi-Agró, Alessandro, Rosenfeld, Ron G., Federici, Giorgio
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blotting, Western
Carrier Proteins - metabolism
Cell Differentiation - drug effects
Down-Regulation
Humans
Immunosorbent Techniques
Insulin-Like Growth Factor Binding Protein 2
Insulin-Like Growth Factor Binding Protein 4
Insulin-Like Growth Factor Binding Proteins
insulin-like growth factors
Medical sciences
Neurites - drug effects
neuroblastoma
Neuroblastoma - metabolism
Neuroblastoma - ultrastructure
Neurology
retinoic acid
Tretinoin - pharmacology
Tumor Cells, Cultured
Tumors of the nervous system. Phacomatoses
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Summary:Insulin‐like growth factors (IGFs) regulate the autocrine/paracrine growth of neuroblastomas. The IGFs bind to specific binding proteins (IGFBPs) which modulate their biological activity. We investigated, by Western ligand blotting (WLB), the presence of IGFBPs and their possible modulation by retinoic acid (RA), IGF‐I, IGF‐II and truncated Des(1‐3)IGF‐l in conditioned medium (CM) of the human neuroblastoma SK‐N‐BE(2) cell line. We demonstrated the presence of two IGFBPs, with MW 37 kDa and 25 kDa. Following immunoprecipitation, they turned out to be IGFBP‐2 and ‐4, respectively. The RA‐induced differentiation in SK‐N‐BE(2) cells was accompanied by a marked reduction of the intensity of both IGFBP bands after 48 h (32% and 24% of control, respectively) and 72 h (2% and 0% of control, respectively) incubation. The addition of exogenous IGFs, which did not induce cell differentiation, did not change the IGFBP pattern significantly, except for the truncated form of IGF‐I, which induced a marked decrease in both the 37 kDa and 25kDa bands after 72 h incubation (45% and 18% of control, respectively). These findings suggest that IGFBPs have a role in RA‐induced differentiation in human neuroblastoma cells.
ISSN:0953-8194
1365-2826
DOI:10.1111/j.1365-2826.1994.tb00601.x