In-vitro antimicrobial susceptibility and β-lactamases of ampicillin-resistant Escherichia coli in Hong Kong

Three hundred and forty isolates of ampicillin-resistant Escherichia coli isolated from blood, bile and urine were collected during 1984 and 1988 in the Prince of Wales Hospital, Hong Kong and MICs of 21 beta-lactam antibiotics were determined. beta-Lactamases were identified by isoelectric focusing...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 1994-07, Vol.34 (1), p.65-71
Main Authors: LING, T. K. W, LYON, D. J, CHENG, A. F. B, FRENCH, G. L
Format: Article
Language:English
Subjects:
Ampicillin Resistance
Anti-Bacterial Agents - pharmacology
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Base Sequence
beta-Lactamases - biosynthesis
beta-Lactamases - genetics
beta-Lactamases - metabolism
beta-Lactams
Biological and medical sciences
DNA, Bacterial - analysis
Escherichia coli - drug effects
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli Infections - microbiology
Fundamental and applied biological sciences. Psychology
Hong Kong
Humans
Isoelectric Focusing
Isoenzymes - genetics
Isoenzymes - metabolism
Metabolism. Enzymes
Microbial Sensitivity Tests
Microbiology
Molecular Sequence Data
Nucleic Acid Hybridization
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Summary:Three hundred and forty isolates of ampicillin-resistant Escherichia coli isolated from blood, bile and urine were collected during 1984 and 1988 in the Prince of Wales Hospital, Hong Kong and MICs of 21 beta-lactam antibiotics were determined. beta-Lactamases were identified by isoelectric focusing, and TEM-1 was found in 89.1% of isolates, PSE-1 in 3.2%, and OXA-1 in 2.9% of strains. Ten isolates (2.9%) possessed both TEM-1 and PSE-1. Five isolates (1.5%) produced only chromosomal enzymes and one produced an unidentified enzyme which focussed at pI 6.0. All but seven of 274 TEM-1 producers and all nine OXA-1 producers reacted with oligonucleotide probes in a colony blot technique. All isolates contained plasmids and 229 of 318 (72.0%) isolates transferred the ampicillin resistance factor. Of 42 transconjugants of TEM-1 producers selected for study, 39(92.9%) had only a single transferable plasmid bearing the TEM-1 gene.
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/34.1.65