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protein involved in co-ordinated regulation of inorganic carbon and glucose metabolism in the facultative photoautotrophic cyanobacterium Synechocystis PCC6803
The involvement of a gene of Synechocystis PCC6803, icfG, in the co-ordinated regulation of inorganic carbon and glucose metabolism, was established. The icfG gene codes for a 72 kDa protein, which shows no homology with those registered in data libraries. Expression of icfG required glucose, the ac...
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| Published in: | Plant molecular biology 1994-08, Vol.25 (5), p.855-864 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 864 |
| container_issue | 5 |
| container_start_page | 855 |
| container_title | Plant molecular biology |
| container_volume | 25 |
| creator | Beuf, L Bedu, S Durand, M.C Joset, F |
| description | The involvement of a gene of Synechocystis PCC6803, icfG, in the co-ordinated regulation of inorganic carbon and glucose metabolism, was established. The icfG gene codes for a 72 kDa protein, which shows no homology with those registered in data libraries. Expression of icfG required glucose, the actual inducer probably being glucose-6-phosphate, and was independent of light and of the external inorganic carbon concentration. Mutants carrying an inactivated copy of icfG were constructed. Their growth characteristics were identical to those of the wild type under all regimes except in limiting inorganic carbon with glucose being present either before or after the transfer to the limiting conditions. These conditions completely prevented growth, both in the light and in the dark. The inhibition could be relieved by several intermediates of the tricarboxylic acid cycle. Assays of various enzymic activities related to inorganic carbon uptake and to its assimilation via either the Calvin cycle or phosphoenolpyruvate carboxylase did not reveal the level of action of IcfG. Possible models include a blockage of the assimilation of both carbon sources in the absence of Icfg or the inhibition of Ci incorporation route(s) essential under limiting inorganic carbon conditions, even when glucose is present, and even in the dark. |
| doi_str_mv | 10.1007/BF00028880 |
| format | article |
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The icfG gene codes for a 72 kDa protein, which shows no homology with those registered in data libraries. Expression of icfG required glucose, the actual inducer probably being glucose-6-phosphate, and was independent of light and of the external inorganic carbon concentration. Mutants carrying an inactivated copy of icfG were constructed. Their growth characteristics were identical to those of the wild type under all regimes except in limiting inorganic carbon with glucose being present either before or after the transfer to the limiting conditions. These conditions completely prevented growth, both in the light and in the dark. The inhibition could be relieved by several intermediates of the tricarboxylic acid cycle. Assays of various enzymic activities related to inorganic carbon uptake and to its assimilation via either the Calvin cycle or phosphoenolpyruvate carboxylase did not reveal the level of action of IcfG. Possible models include a blockage of the assimilation of both carbon sources in the absence of Icfg or the inhibition of Ci incorporation route(s) essential under limiting inorganic carbon conditions, even when glucose is present, and even in the dark.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/BF00028880</identifier><identifier>PMID: 8075401</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Adaptation, Biological - genetics ; Amino Acid Sequence ; amino acid sequences ; Bacteria - genetics ; Bacteria - metabolism ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; Base Sequence ; beta-galactosidase ; Calvin cycle ; carbohydrate metabolism ; Carbon Dioxide - metabolism ; Citric Acid Cycle - physiology ; Cloning, Molecular ; Cyanobacteria ; Cyanobacteria - genetics ; Cyanobacteria - growth & development ; Cyanobacteria - metabolism ; genbank/x75568 ; gene expression ; Gene Expression Regulation, Bacterial - drug effects ; Genetic Complementation Test ; glucose ; Glucose - metabolism ; Glucose - pharmacology ; icfg gene ; messenger RNA ; Molecular Sequence Data ; nucleotide sequences ; Phosphoenolpyruvate Carboxylase - analysis ; photosynthesis ; Photosynthesis - physiology ; promoter regions ; proteins ; recombinant DNA ; regulation ; reporter genes ; Restriction Mapping ; structural genes</subject><ispartof>Plant molecular biology, 1994-08, Vol.25 (5), p.855-864</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8075401$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Beuf, L</creatorcontrib><creatorcontrib>Bedu, S</creatorcontrib><creatorcontrib>Durand, M.C</creatorcontrib><creatorcontrib>Joset, F</creatorcontrib><title>protein involved in co-ordinated regulation of inorganic carbon and glucose metabolism in the facultative photoautotrophic cyanobacterium Synechocystis PCC6803</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>The involvement of a gene of Synechocystis PCC6803, icfG, in the co-ordinated regulation of inorganic carbon and glucose metabolism, was established. The icfG gene codes for a 72 kDa protein, which shows no homology with those registered in data libraries. Expression of icfG required glucose, the actual inducer probably being glucose-6-phosphate, and was independent of light and of the external inorganic carbon concentration. Mutants carrying an inactivated copy of icfG were constructed. Their growth characteristics were identical to those of the wild type under all regimes except in limiting inorganic carbon with glucose being present either before or after the transfer to the limiting conditions. These conditions completely prevented growth, both in the light and in the dark. The inhibition could be relieved by several intermediates of the tricarboxylic acid cycle. Assays of various enzymic activities related to inorganic carbon uptake and to its assimilation via either the Calvin cycle or phosphoenolpyruvate carboxylase did not reveal the level of action of IcfG. Possible models include a blockage of the assimilation of both carbon sources in the absence of Icfg or the inhibition of Ci incorporation route(s) essential under limiting inorganic carbon conditions, even when glucose is present, and even in the dark.</description><subject>Adaptation, Biological - genetics</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Bacteria - genetics</subject><subject>Bacteria - metabolism</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>Base Sequence</subject><subject>beta-galactosidase</subject><subject>Calvin cycle</subject><subject>carbohydrate metabolism</subject><subject>Carbon Dioxide - metabolism</subject><subject>Citric Acid Cycle - physiology</subject><subject>Cloning, Molecular</subject><subject>Cyanobacteria</subject><subject>Cyanobacteria - genetics</subject><subject>Cyanobacteria - growth & development</subject><subject>Cyanobacteria - metabolism</subject><subject>genbank/x75568</subject><subject>gene expression</subject><subject>Gene Expression Regulation, Bacterial - drug effects</subject><subject>Genetic Complementation Test</subject><subject>glucose</subject><subject>Glucose - metabolism</subject><subject>Glucose - pharmacology</subject><subject>icfg gene</subject><subject>messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Phosphoenolpyruvate Carboxylase - analysis</subject><subject>photosynthesis</subject><subject>Photosynthesis - physiology</subject><subject>promoter regions</subject><subject>proteins</subject><subject>recombinant DNA</subject><subject>regulation</subject><subject>reporter genes</subject><subject>Restriction Mapping</subject><subject>structural genes</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNotUMFO3DAUtKoiWCiX3it86i3Udhzbe2xXpSAhFYlyjp6dl11Xib21nZX2a_jVGrGn9-bNvNFoCPnM2S1nTH_7cccYE8YY9oGseKfbpqvwI1kxrnQjJRcX5DLnv4xVeavOyblhupOMr8jrPsWCPlAfDnE64FAX6mIT0-ADlIoTbpcJio-BxrGyMW0heEcdJFtvEAa6nRYXM9IZC9g4-Ty_uZQd0hHcMpX6fUC638USYSmxpLjfvTkcIUQLrmDyy0yfjwHdLrpjLj7Tp81GGdZ-ImcjTBmvT_OKvNz9_LO5bx5__3rYfH9sRi670pjBSumEMp1ay9YYa9ZSj4prtsZhMDB2SltjQVhUI9dcG9cJkC1aoZyQ6_aKfH33rX38WzCXfvbZ4TRBwLjkXquaxghehV9OwsXOOPT75GdIx_7UaOVv3vkRYg_b5HP_8iwqwbismbhu_wPc_YOs</recordid><startdate>199408</startdate><enddate>199408</enddate><creator>Beuf, L</creator><creator>Bedu, S</creator><creator>Durand, M.C</creator><creator>Joset, F</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199408</creationdate><title>protein involved in co-ordinated regulation of inorganic carbon and glucose metabolism in the facultative photoautotrophic cyanobacterium Synechocystis PCC6803</title><author>Beuf, L ; Bedu, S ; Durand, M.C ; Joset, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f145t-8db44c2685694388b8947f61709edd8af567b8ba2be6f17178c52a43eb26c2493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Adaptation, Biological - genetics</topic><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Bacteria - genetics</topic><topic>Bacteria - metabolism</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacterial Proteins - genetics</topic><topic>Base Sequence</topic><topic>beta-galactosidase</topic><topic>Calvin cycle</topic><topic>carbohydrate metabolism</topic><topic>Carbon Dioxide - metabolism</topic><topic>Citric Acid Cycle - physiology</topic><topic>Cloning, Molecular</topic><topic>Cyanobacteria</topic><topic>Cyanobacteria - genetics</topic><topic>Cyanobacteria - growth & development</topic><topic>Cyanobacteria - metabolism</topic><topic>genbank/x75568</topic><topic>gene expression</topic><topic>Gene Expression Regulation, Bacterial - drug effects</topic><topic>Genetic Complementation Test</topic><topic>glucose</topic><topic>Glucose - metabolism</topic><topic>Glucose - pharmacology</topic><topic>icfg gene</topic><topic>messenger RNA</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Phosphoenolpyruvate Carboxylase - analysis</topic><topic>photosynthesis</topic><topic>Photosynthesis - physiology</topic><topic>promoter regions</topic><topic>proteins</topic><topic>recombinant DNA</topic><topic>regulation</topic><topic>reporter genes</topic><topic>Restriction Mapping</topic><topic>structural genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Beuf, L</creatorcontrib><creatorcontrib>Bedu, S</creatorcontrib><creatorcontrib>Durand, M.C</creatorcontrib><creatorcontrib>Joset, F</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Beuf, L</au><au>Bedu, S</au><au>Durand, M.C</au><au>Joset, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>protein involved in co-ordinated regulation of inorganic carbon and glucose metabolism in the facultative photoautotrophic cyanobacterium Synechocystis PCC6803</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1994-08</date><risdate>1994</risdate><volume>25</volume><issue>5</issue><spage>855</spage><epage>864</epage><pages>855-864</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>The involvement of a gene of Synechocystis PCC6803, icfG, in the co-ordinated regulation of inorganic carbon and glucose metabolism, was established. The icfG gene codes for a 72 kDa protein, which shows no homology with those registered in data libraries. Expression of icfG required glucose, the actual inducer probably being glucose-6-phosphate, and was independent of light and of the external inorganic carbon concentration. Mutants carrying an inactivated copy of icfG were constructed. Their growth characteristics were identical to those of the wild type under all regimes except in limiting inorganic carbon with glucose being present either before or after the transfer to the limiting conditions. These conditions completely prevented growth, both in the light and in the dark. The inhibition could be relieved by several intermediates of the tricarboxylic acid cycle. Assays of various enzymic activities related to inorganic carbon uptake and to its assimilation via either the Calvin cycle or phosphoenolpyruvate carboxylase did not reveal the level of action of IcfG. Possible models include a blockage of the assimilation of both carbon sources in the absence of Icfg or the inhibition of Ci incorporation route(s) essential under limiting inorganic carbon conditions, even when glucose is present, and even in the dark.</abstract><cop>Netherlands</cop><pmid>8075401</pmid><doi>10.1007/BF00028880</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 1994-08, Vol.25 (5), p.855-864 |
| issn | 0167-4412 1573-5028 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76680821 |
| source | Springer Archives (Through 1996) |
| subjects | Adaptation, Biological - genetics Amino Acid Sequence amino acid sequences Bacteria - genetics Bacteria - metabolism Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Base Sequence beta-galactosidase Calvin cycle carbohydrate metabolism Carbon Dioxide - metabolism Citric Acid Cycle - physiology Cloning, Molecular Cyanobacteria Cyanobacteria - genetics Cyanobacteria - growth & development Cyanobacteria - metabolism genbank/x75568 gene expression Gene Expression Regulation, Bacterial - drug effects Genetic Complementation Test glucose Glucose - metabolism Glucose - pharmacology icfg gene messenger RNA Molecular Sequence Data nucleotide sequences Phosphoenolpyruvate Carboxylase - analysis photosynthesis Photosynthesis - physiology promoter regions proteins recombinant DNA regulation reporter genes Restriction Mapping structural genes |
| title | protein involved in co-ordinated regulation of inorganic carbon and glucose metabolism in the facultative photoautotrophic cyanobacterium Synechocystis PCC6803 |
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