protein involved in co-ordinated regulation of inorganic carbon and glucose metabolism in the facultative photoautotrophic cyanobacterium Synechocystis PCC6803

The involvement of a gene of Synechocystis PCC6803, icfG, in the co-ordinated regulation of inorganic carbon and glucose metabolism, was established. The icfG gene codes for a 72 kDa protein, which shows no homology with those registered in data libraries. Expression of icfG required glucose, the ac...

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Bibliographic Details
Published in:Plant molecular biology 1994-08, Vol.25 (5), p.855-864
Main Authors: Beuf, L, Bedu, S, Durand, M.C, Joset, F
Format: Article
Language:English
Subjects:
Adaptation, Biological - genetics
Amino Acid Sequence
amino acid sequences
Bacteria - genetics
Bacteria - metabolism
Bacterial Proteins - biosynthesis
Bacterial Proteins - genetics
Base Sequence
beta-galactosidase
Calvin cycle
carbohydrate metabolism
Carbon Dioxide - metabolism
Citric Acid Cycle - physiology
Cloning, Molecular
Cyanobacteria
Cyanobacteria - genetics
Cyanobacteria - growth & development
Cyanobacteria - metabolism
genbank/x75568
gene expression
Gene Expression Regulation, Bacterial - drug effects
Genetic Complementation Test
glucose
Glucose - metabolism
Glucose - pharmacology
icfg gene
messenger RNA
Molecular Sequence Data
nucleotide sequences
Phosphoenolpyruvate Carboxylase - analysis
photosynthesis
Photosynthesis - physiology
promoter regions
proteins
recombinant DNA
regulation
reporter genes
Restriction Mapping
structural genes
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Summary:The involvement of a gene of Synechocystis PCC6803, icfG, in the co-ordinated regulation of inorganic carbon and glucose metabolism, was established. The icfG gene codes for a 72 kDa protein, which shows no homology with those registered in data libraries. Expression of icfG required glucose, the actual inducer probably being glucose-6-phosphate, and was independent of light and of the external inorganic carbon concentration. Mutants carrying an inactivated copy of icfG were constructed. Their growth characteristics were identical to those of the wild type under all regimes except in limiting inorganic carbon with glucose being present either before or after the transfer to the limiting conditions. These conditions completely prevented growth, both in the light and in the dark. The inhibition could be relieved by several intermediates of the tricarboxylic acid cycle. Assays of various enzymic activities related to inorganic carbon uptake and to its assimilation via either the Calvin cycle or phosphoenolpyruvate carboxylase did not reveal the level of action of IcfG. Possible models include a blockage of the assimilation of both carbon sources in the absence of Icfg or the inhibition of Ci incorporation route(s) essential under limiting inorganic carbon conditions, even when glucose is present, and even in the dark.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00028880