Recognition of novel and divergent higher plant chloroplast ribosomal proteins by Escherichia coli ribosome during in vivo assembly

Architecture of higher plant chloroplast ribosomes involves additional protein domains over that found in the Escherichia coli ribosome, although the rRNAs in these two kinds of ribosomes are very similar in length and sequence (Subramanian, A. R. (1993) Trends Biochem. Sci. 18, 177-180). Here, we s...

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Bibliographic Details
Published in:The Journal of biological chemistry 1994-07, Vol.269 (27), p.18223-18231
Main Authors: Bubunenko, M.G. (Max-Planck-Institut fur Molekulare Genetik, Berlin-Dahlem, Germany.), Subramanian, A.R
Format: Article
Language:English
Subjects:
ADN
Base Sequence
Biological and medical sciences
Cell structures and functions
CHLOROPLASTE
Chloroplasts - metabolism
Cloning, Molecular
CLOROPLASTO
DNA
ESCHERICHIA COLI
Escherichia coli - growth & development
Escherichia coli - metabolism
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Molecular Sequence Data
Plant Proteins - metabolism
Plants
PROTEINAS
PROTEINE
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Ribosomal Proteins - metabolism
RIBOSOMAS
RIBOSOME
Ribosomes - metabolism
SPINACIA OLERACEA
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Summary:Architecture of higher plant chloroplast ribosomes involves additional protein domains over that found in the Escherichia coli ribosome, although the rRNAs in these two kinds of ribosomes are very similar in length and sequence (Subramanian, A. R. (1993) Trends Biochem. Sci. 18, 177-180). Here, we show that two chloroplast-specific protein domains (a novel chloroplast ribosomal protein of the 30 S subunit, called Psrp-1 or S22, and a divergent protein of the 50 S subunit with long terminal extensions and low homology to its E. coli counterpart, L21) are both incorporated in E. coli ribosomes and polysomes when their gene constructs are expressed in E. coli. Also, the 67-residue NH2-terminal extension in chloroplast L21 by itself is incorporated. Thus, our results indicate preexisting binding sites for novel chloroplast-specific ribosomal proteins/domains on eubacterial ribosomes. Additionally, we observed cleavage of the chloroplast-targeting transit peptide (present in the expressed Psrp-1 precursor), indicating protease(s) of the required specificity in E. coli cells. The expression of chloroplast L21 with its NH2-terminal extension was inhibitory to E. coli growth, suggesting a drastic effect of the latter on some property of L21. Expression of Psrp-1 was neutral, consistent with a function only in chloroplast translation. Based on analysis of the assembly of Psrp-1 and various L21 fragments in E. coli ribosomes, a general model for studying ribosomal protein-ribosome interactions is suggested
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)32438-9