A Reliable Method of Embedding a Small Amount of Dispersed Cells for Electron Microscopy

To embed a small amount of dispersed cells for electron microscopy, we used the supernatant of agarose solution which had been centrifuged beforehand. We also wanned the chamber of the centrifuge by placing a flexible ribbon heater at the bottom of the chamber to prevent the premature gelation of ag...

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Bibliographic Details
Published in:Journal of electron microscopy 1994-02, Vol.43 (1), p.48-50
Main Authors: Taniguchi, Yutaka, Tamatani, Rieko, Kawarai, Yasuyuki
Format: Article
Language:English
Subjects:
agarose
Biological and medical sciences
Cells, Cultured - ultrastructure
dispersed cells
Diverse techniques
embedding method
Fundamental and applied biological sciences. Psychology
Microscopy, Electron - methods
Molecular and cellular biology
Sepharose
TEM
Tissue Embedding - methods
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Summary:To embed a small amount of dispersed cells for electron microscopy, we used the supernatant of agarose solution which had been centrifuged beforehand. We also wanned the chamber of the centrifuge by placing a flexible ribbon heater at the bottom of the chamber to prevent the premature gelation of agarose. By these methods, as few as 106 dispersed cells can be reproducibly processed and embedded for electron microscopy.
ISSN:0022-0744
1477-9986
1477-9986
DOI:10.1093/oxfordjournals.jmicro.a051080