Coproantigen detection in rats experimentally infected with Strongyloides ratti

The possibility of coproantigen detection in the diagnosis of strongyloidiasis was investigated in rats experimentally infected with Strongyloides ratti. A capture ELISA was developed using affinity-purified polyclonal antibodies raised in rabbits against adult and filariform larval somatic antigens...

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Bibliographic Details
Published in:Parasitology 1994-04, Vol.108 (3), p.335-342
Main Authors: Nageswaran, C., Craig, P. S., Devaney, E.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Helminth - biosynthesis
Antigens, Helminth - analysis
Antigens, Helminth - immunology
Biological and medical sciences
coproantigen detection
Enzyme-Linked Immunosorbent Assay
experimental infection
Feces - parasitology
Helminthic diseases
humoral response
Immunoglobulin G - biosynthesis
Immunoglobulin M - biosynthesis
Infectious diseases
Medical sciences
Miscellaneous
Parasite Egg Count
Parasitic diseases
Rats
Rats, Sprague-Dawley
Sensitivity and Specificity
Strongyloides ratti
Strongyloides ratti - immunology
Strongyloidiasis - diagnosis
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Summary:The possibility of coproantigen detection in the diagnosis of strongyloidiasis was investigated in rats experimentally infected with Strongyloides ratti. A capture ELISA was developed using affinity-purified polyclonal antibodies raised in rabbits against adult and filariform larval somatic antigens. The first detection of coproantigen coincided with patency and the coproantigen level remained elevated even when the parasite count was low. Following challenge infection, when examination of stool for larvae or ova was negative, coproantigen was detected by anti-adult somatic capture ELISA. Despite the use of antisera raised against crude worm extracts, the test was specific showing only low cross-reactivity with Necator americanus and Syphacia muris.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182000076174