Molecular cloning of mannose-binding lectins from Clivia miniata

Screening of a cDNA library constructed from total RNA isolated from young developing ovaries of Clivia miniata Regel with the amaryllis lectin cDNA clone resulted in the isolation of four different isolectin clones which clearly differ from each other in their nucleotide sequences and hence also in...

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Bibliographic Details
Published in:Plant molecular biology 1994-03, Vol.24 (5), p.825-830
Main Authors: Damme, E.J.M. Van (Leuven Catholic Univ. (Belgium). Lab. for Phytopathology and Plant Protection), Smeets, K, Leuven, F. Van, Peumans, W.J
Format: Article
Language:English
Subjects:
AMARYLLIDACEAE
Amino Acid Sequence
Base Sequence
Biological and medical sciences
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
Cloning, Molecular
DNA, Complementary - genetics
Fundamental and applied biological sciences. Psychology
Genes. Genome
LECTINA
LECTINE
LECTINS
Lectins - genetics
Lectins - isolation & purification
MANNOSE
Mannose - metabolism
MANOSA
Molecular and cellular biology
MOLECULAR CLONING
Molecular genetics
Molecular Sequence Data
Plant Lectins
Plants - genetics
RNA, Messenger - analysis
Sequence Analysis
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
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Summary:Screening of a cDNA library constructed from total RNA isolated from young developing ovaries of Clivia miniata Regel with the amaryllis lectin cDNA clone resulted in the isolation of four different isolectin clones which clearly differ from each other in their nucleotide sequences and hence also in their deduced amino acid sequences. Apparently the lectin is translated from an mRNA of ca. 800 nucleotides encoding a precursor polypeptide of 163 amino acids. Northern blot analysis of total RNA isolated from different tissues of Clivia miniata has shown that the lectin is expressed in most plant tissues with very high lectin mRNA concentrations in the ovary and the seed endosperm.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00029865