Vascular Free Radical Release: Ex Vivo and In Vivo Evidence for a Flow-Dependent Endothelial Mechanism

Mechanisms underlying production of vascular free radicals are unclear. We hypothesized that changes in blood flow might serve as a physiological stimulus for endothelial free radical release. Intact isolated aortas from 45 rabbits were perfused with the spin trap α-phenyl-N-tert-butylnitrone (PBN,...

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Bibliographic Details
Published in:Circulation research 1994-04, Vol.74 (4), p.700-709
Main Authors: Martins Laurindo, Francisco Rafael, de Almeida Pedro, Marcelo, Barbeiro, Hermes Vieira, Pileggi, Fuilvio, Catelli Carvalho, Maria Helena, Augusto, Ohara, da Luz, Protásio Lemos
Format: Article
Language:English
Subjects:
Acetylcholine - pharmacology
Amino Acid Oxidoreductases - physiology
Animals
Ascorbic Acid - metabolism
Biological and medical sciences
Electron Spin Resonance Spectroscopy
Endothelium, Vascular - metabolism
Free Radicals
Fundamental and applied biological sciences. Psychology
Hemodynamics
Hemodynamics. Rheology
Indomethacin - pharmacology
Male
Nitric Oxide Synthase
Perfusion
Rabbits
Regional Blood Flow
Superoxide Dismutase - pharmacology
Vertebrates: cardiovascular system
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Summary:Mechanisms underlying production of vascular free radicals are unclear. We hypothesized that changes in blood flow might serve as a physiological stimulus for endothelial free radical release. Intact isolated aortas from 45 rabbits were perfused with the spin trap α-phenyl-N-tert-butylnitrone (PBN, 20 mmol/L) and formed radical adducts detected by electron paramagnetic resonance spectroscopy (EPR). Sequential perfusion at 2, 7.5, and 12 mL/min changed cumulative vascular PBN radical adduct yields, respectively, from 3.2±0.9 to 4.1±0.7 (P
ISSN:0009-7330
1524-4571
DOI:10.1161/01.res.74.4.700