Crystallization and Preliminary X-Ray Studies on Pseudoazurin from Achromobacter cycloclastes IAM1013

New crystals of a blue copper protein, pseudoazurin from denitrlfier Achromobacter cycloclastes IAM1013, have been obtained by means of vapor diffusion with ammonium sulfate as a precipitant at pH 6.0 and 4°C. The crystals belong to the orthorhombic system, space group P212121, with unit cell dimens...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1993-12, Vol.114 (6), p.761-762
Main Authors: Inoue, Tsuyoshi, Nishio, Nobuya, Kai, Yasushi, Harada, Shigehara, Ohshiro, Yoshiki, Suzuki, Shinnichiro, Kohzuma, Takamitsu, Shidara, Sousuke, Iwasaki, Hidekazu
Format: Article
Language:English
Subjects:
Alcaligenes - chemistry
Azurin - analogs & derivatives
Azurin - chemistry
Azurin - isolation & purification
Biological and medical sciences
Crystalline structure
Crystallography, X-Ray
Fundamental and applied biological sciences. Psychology
Molecular biophysics
Structure in molecular biology
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Summary:New crystals of a blue copper protein, pseudoazurin from denitrlfier Achromobacter cycloclastes IAM1013, have been obtained by means of vapor diffusion with ammonium sulfate as a precipitant at pH 6.0 and 4°C. The crystals belong to the orthorhombic system, space group P212121, with unit cell dimensions of a=56.69(2), b=61.53(2), and c = 30.20(l) Å. The asymmetric unit includes one molecule of pseudoazurin with a Vm value of 2.04 Å3/ Da. The crystals are so stable against X-ray irradiation that a complete data set up to 1.54 Å has been collected using a single native crystal. Solution of the structure was performed by means of the Patterson search techniques, and the current crystallographic R-factor is 17.5% at 3.0 Å resolution. Refinement at higher resolution is in progress.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a124251