Dynamics in cytokine responses during the development of occupational sensitization to rats

To cite this article: Krop EJM, van de Pol MA, Lutter R, Heederik DJJ, Aalberse RC, van der Zee JS. Dynamics in cytokine responses during the development of occupational sensitization to rats. Allergy 2010; 65: 1227-1233. Occupational allergy forms an attractive model to study the development of all...

Full description

Saved in:
Bibliographic Details
Published in:Allergy (Copenhagen) 2010-10, Vol.65 (10), p.1227-1233
Main Authors: Krop, E.J.M, van de Pol, M.A, Lutter, R, Heederik, D.J.J, Aalberse, R.C, van der Zee, J.S
Format: Article
Language:English
Subjects:
Allergens
Allergies
Animal models
Animal Technicians
Animals
Atopy
Biological and medical sciences
Case-Control Studies
Cytokines
Cytokines - blood
Cytokines - immunology
Dermatology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
g-Interferon
gamma -Interferon
Humans
Hypersensitivity
Hypersensitivity - etiology
Hypersensitivity - immunology
IgE
IL-4
Immunoglobulin E
Immunoglobulin E - blood
Immunoglobulins
Interleukin 10
Interleukin 4
Interleukin-4 - blood
Interleukin-4 - immunology
laboratory animal allergy
Laboratory animals
Medical Laboratory Personnel
Medical sciences
occupational allergy
Occupational diseases
Occupational Diseases - etiology
Occupational Diseases - immunology
Occupational Exposure - adverse effects
Peripheral blood
Rats - immunology
Researchers
Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
Th2 responses
Workers
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To cite this article: Krop EJM, van de Pol MA, Lutter R, Heederik DJJ, Aalberse RC, van der Zee JS. Dynamics in cytokine responses during the development of occupational sensitization to rats. Allergy 2010; 65: 1227-1233. Occupational allergy forms an attractive model to study the development of allergic responses, as in some occupations it has a high incidence and develops quickly. In a cohort of starting laboratory animal workers, we previously found 20% sensitization to animal allergens within 2 years. We compared cellular responses of incident laboratory animal workers who developed rat-specific sensitization (cases, n = 18) during 2 years of follow-up to control animal workers matched for atopic status but without sensitization after follow-up (controls, n = 18). Practically, this is a case-control study, nested within the cohort. Rat-specific IgE antibodies were measured in sera, and allergen-specific and nonspecific cytokine responses were measured in whole blood and in isolated peripheral blood mononuclear cells. Self-reported allergic symptoms were related to the presence of rat-specific IgE (P [less-than or equal to] 0.01). Cases developed a rat allergen-specific interleukin (IL)-4 response during sensitization, while controls did not show an increased IL-4 response (at visit D: 33 vs 5 IL-4 producing cells/10⁶ cells, P < 0.001). The IL-4 response was related to the levels of rat-specific IgE in cases (visit D: rho = 0.706, P < 0.001). By contrast, allergen-specific IL-10 and interferon γ (IFNγ) responses as well as nonspecific cytokine responses were comparable between cases and controls. This study is the first to show the development of an allergen-specific IL-4 response in adult human subjects during allergen-specific sensitization. This IL-4 response was quantitatively associated with the development of the specific IgE antibodies. Allergen-specific or nonspecific IL-10 and IFNγ responses showed no protective effect on the development of allergic sensitization.
ISSN:0105-4538
1398-9995
DOI:10.1111/j.1398-9995.2009.02323.x