Beauvericin and ochratoxin A genotoxicity evaluated using the alkaline comet assay: single and combined genotoxic action

This study was aimed at investigating the genotoxic potential of single beauvericin (BEA) and ochratoxin A (OTA) as well as their interaction in porcine kidney epithelial PK15 cells and human leukocytes using the alkaline comet assay. IC 50 of BEA (5.0 ± 0.6) and OTA (15.8 ± 1.5) estimated by MTT re...

Full description

Saved in:
Bibliographic Details
Published in:Archives of toxicology 2010-08, Vol.84 (8), p.641-650
Main Authors: Klarić, Maja Šegvić, Daraboš, Dina, Rozgaj, Ružica, Kašuba, Vilena, Pepeljnjak, Stjepan
Format: Article
Language:English
Subjects:
Animals
Bioassays
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cell Line
Cell Survival - drug effects
Cellular biology
Comet Assay
Depsipeptides - toxicity
Drug Interactions
Environmental Health
Genotoxicity and Carcinogenicity
Genotype & phenotype
Humans
Kidney - drug effects
Kidney - metabolism
Leukocytes
Leukocytes - drug effects
Leukocytes - metabolism
Medical sciences
Metabolites
Mutagens - toxicity
Occupational Medicine/Industrial Medicine
Ochratoxins - toxicity
Pharmacology/Toxicology
Plant poisons toxicology
Swine
Toxicity Tests
Toxicology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study was aimed at investigating the genotoxic potential of single beauvericin (BEA) and ochratoxin A (OTA) as well as their interaction in porcine kidney epithelial PK15 cells and human leukocytes using the alkaline comet assay. IC 50 of BEA (5.0 ± 0.6) and OTA (15.8 ± 1.5) estimated by MTT reduction assay shows that BEA is three times more toxic than OTA. BEA (0.1 and 0.5 μM) and OTA (1 and 5 μM) were applied alone or in combination of these concentrations for 1 and 24 h in PK15 cells and human leukocytes. Genotoxicity of these toxins to PK15 cells was time- and concentration dependent. After 1 h, significant increase in tail length, tail intensity, tail moment, and abnormal sized tails (AST) was noted upon exposure to 1 μM of OTA alone and BEA + OTA combinations. Single BEA (0.5 μM) and OTA (1 and 5 μM) and their combinations evoked significant DNA damage in PK15 cells, considering all comet tail parameters measured after 24 h of treatment. Human leukocytes were slightly concentration but not time dependent. After 1 h of exposure, there were no significant changes in the tail length. Tail intensity, tail moment, and/or incidence of AST were significantly higher in cells treated with single OTA or BEA and their combinations than in control cells. DNA damage in leukocytes was significantly higher after 24 h of exposure to single toxins and their combinations, considering all comet tail parameters, but these changes were less pronounced than in PK15 cells. Combined toxins showed additive and synergistic effects in PK15 cells, while only additive effects were observed in human leukocytes. Combined prolonged exposure to BEA and OTA in subcytotoxic concentrations through food consumption could induce DNA damage contributing to the carcinogenicity in animals and humans.
ISSN:0340-5761
1432-0738
DOI:10.1007/s00204-010-0535-7