Cell numbers and ploidy classes in the normal bladder urothelium in hairless mice: based on an improved method for dispersion and preparation of cells for flow cytometry

A simple method for obtaining high-yield single cell suspensions from mouse urothelium is reported. The yield of cells per bladder is about 5 X 10(5), or more than twice that of previously published methods. The method gives a satisfactory coefficient of variation and regularly low levels of debris...

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Bibliographic Details
Published in:Virchows Archiv. Abteilung B. Zellpathologie 1985, Vol.48 (4), p.289-297
Main Author: REITAN, J. B
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Count
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Male
Mice
Mice, Hairless
Mice, Inbred BALB C
Ploidies
Urinary Bladder - cytology
Vertebrates: urinary system
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Summary:A simple method for obtaining high-yield single cell suspensions from mouse urothelium is reported. The yield of cells per bladder is about 5 X 10(5), or more than twice that of previously published methods. The method gives a satisfactory coefficient of variation and regularly low levels of debris when used for flow cytometry.
ISSN:0340-6075
0042-6431
DOI:10.1007/BF02890136