The effect of glucagon and adrenergic agonists on intracellular pH of isolated rat hepatocytes

19F NMR was used to measure intracellular [H+] of hepatocytes before and after incubation with glucagon and adrenergic agonists at their concentrations which give maximal stimulation of both glucose and urea production. Intracellular and extracellular pH was determined from the chemical shifts in re...

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Bibliographic Details
Published in:The Journal of biological chemistry 1985-06, Vol.260 (11), p.6808-6810
Main Authors: Deutsch, C J, Kashiwagura, T, Taylor, J, Wilson, D F, Erecińska, M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bucladesine - pharmacology
Cell physiology
Fundamental and applied biological sciences. Psychology
Glucagon - pharmacology
Gluconeogenesis - drug effects
Hydrogen-Ion Concentration
Liver - cytology
Liver - drug effects
Magnetic Resonance Spectroscopy
Male
Membrane and intracellular transports
Molecular and cellular biology
Rats
Rats, Inbred Strains
Sympathomimetics - pharmacology
Urea - biosynthesis
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Summary:19F NMR was used to measure intracellular [H+] of hepatocytes before and after incubation with glucagon and adrenergic agonists at their concentrations which give maximal stimulation of both glucose and urea production. Intracellular and extracellular pH was determined from the chemical shifts in resonances of alpha-difluoromethylalanine. The alterations in intracellular [H+] with agonist treatment were, in all cases, found to be less than 0.1 pH unit in the pH range 6.7-7.8. It is concluded that changes in concentration of the intracellular [H+] do not play a significant role in the stimulation of urea and glucose production caused by these hormonal effectors.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)88851-2