A low‐Mr chemoattractant for vascular endothelial cells

The formation of new blood vessels occurs by sprouting from previously existing microvasculature. The process involved directed migration of the vascular endothelial cells towards chemical signals released from the target tissue. We have used the Boyden chemotaxis chamber method to identify chemotac...

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Bibliographic Details
Published in:FEBS letters 1985-05, Vol.184 (2), p.254-258
Main Authors: BUKI, K. G, SEPPA, H
Format: Article
Language:English
Subjects:
Animals
Applied sciences
Biological and medical sciences
Biological Assay
Blood vessels and receptors
Cattle
Chemotactic Factors - isolation & purification
Chemotaxis
Endothelium - cytology
Endothelium - physiology
Exact sciences and technology
Female
Fundamental and applied biological sciences. Psychology
Humans
Molecular Weight
Neovascularization Angiogenesis Vascular endothelial cell Chemotaxis Cell motility Placenta
Neovascularization, Pathologic
Other techniques and industries
Placenta - analysis
Pregnancy
Vertebrates: cardiovascular system
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Summary:The formation of new blood vessels occurs by sprouting from previously existing microvasculature. The process involved directed migration of the vascular endothelial cells towards chemical signals released from the target tissue. We have used the Boyden chemotaxis chamber method to identify chemotactic signals for fetal bovine vascular endothelial cells. Human placenta organ cultures produce a high‐M r chemoattractant for the endothelial cells from which a low‐M r factor can be liberated with trichloroacetic acid treatment and ethanol extraction. This activity was isolated from extracts of human placenta using Sephadex LH‐20, Amberlite XAD‐2, and silica gel thin‐layer chromatography. The M r of the factor is less than 400, it is lipophilic and resistant to proteolytic enzymes. The factor induces chemotactic migration of both aortic endothelial cells and capillary endothelial cells from the retina, but has no effect on fibroblasts or leukocytes suggesting a specific function of the compound for the vascular endothelial cells.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(85)80617-7