Methanosphaera stadtmaniae gen. nov., sp. nov.: a species that forms methane by reducing methanol with hydrogen

Methanosphaera stadtmaniae is a non-motile, Gram-positive spherical-shaped organism that obtains energy for growth by using hydrogen to reduce methanol to methane. It does not produce methane from hydrogen and carbon dioxide, formate, acetate or methylamines and cannot grow with hydrogen and carbon...

Full description

Saved in:
Bibliographic Details
Published in:Archives of microbiology 1985-03, Vol.141 (2), p.116-122
Main Authors: Miller, T.L, Wolin, M.J
Format: Article
Language:English
Subjects:
Bacteriology
BAKTERIEN
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
BIOSYNTHESE
Biotechnology
DNA, Bacterial - analysis
Euryarchaeota - growth & development
Euryarchaeota - isolation & purification
Euryarchaeota - physiology
Fundamental and applied biological sciences. Psychology
Hydrogen - metabolism
Hydrogen-Ion Concentration
Isolation and description
METHAN
Methane - metabolism
METHANE PRODUCTION
METHANOBACTERIACEAE
METHANOL
Methanol - metabolism
Microbiology
Mission oriented research
Nitrogen - metabolism
PRODUCCION DE METANO
PRODUCTION DE METHANE
REDUKTION (CHEMIE)
Systematics
Temperature
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Methanosphaera stadtmaniae is a non-motile, Gram-positive spherical-shaped organism that obtains energy for growth by using hydrogen to reduce methanol to methane. It does not produce methane from hydrogen and carbon dioxide, formate, acetate or methylamines and cannot grow with hydrogen and carbon monoxide, nitrate, fumarate, sulfate or choline. Its pH optimum is 6.5 to 6.9 and its temperature optimum is 36 degrees to 40 degrees C. It is not inhibited by bile salts, inhibitors of the synthesis of folic acid coenzymes, cephalothin or clindamycin but is inhibited by metronidazole, bacitracin, monensin, lasalocid, or bromoethanesulfonate. It requires acetate, carbon dioxide, isoleucine, ammonium, and thiamin for growth and biotin is stimulatory. It does not contain cytochromes and the mol % G + C of its DNA is 25.8. The composition of its cell wall and 16 S rRNA and its immunological fingerprint are consistent with characterization of the organism as a member of a new genus of the family Methanobacteriaceae. The habitat of the type strain is the human large intestine.
ISSN:0302-8933
1432-072X
DOI:10.1007/BF00423270