DNA topoisomerase II and casein kinase II associate in a molecular complex that is catalytically active

Immunoprecipitation of DNA topoisomerase II from yeast results in a preparation that contains casein kinase II; this suggests that the two proteins may associate in the intact cell. Purified recombinant topoisomerase II and casein kinase II associate to form a complex in vitro which is stable after...

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Bibliographic Details
Published in:The Journal of biological chemistry 1993-10, Vol.268 (30), p.22920-22926
Main Authors: Bojanowski, K, Filhol, O, Cochet, C, Chambaz, E.M., Larsen, A.K.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Casein Kinase II
DNA Topoisomerases, Type II - chemistry
DNA Topoisomerases, Type II - metabolism
DNA, Viral - metabolism
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Heparin - pharmacology
Heparitin Sulfate - pharmacology
Isomerases
Kinetics
Macromolecular Substances
Molecular Sequence Data
Moths
Phosphorylation
Protein-Serine-Threonine Kinases - chemistry
Protein-Serine-Threonine Kinases - metabolism
Recombinant Proteins - chemistry
Recombinant Proteins - metabolism
Simian virus 40
Spermine - pharmacology
Substrate Specificity
Suramin - pharmacology
Transfection
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Summary:Immunoprecipitation of DNA topoisomerase II from yeast results in a preparation that contains casein kinase II; this suggests that the two proteins may associate in the intact cell. Purified recombinant topoisomerase II and casein kinase II associate to form a complex in vitro which is stable after topoisomerase II becomes phosphorylated by the kinase. Studies with isolated recombinant casein kinase II subunits disclosed that although the alpha (catalytic) subunit alone can efficiently phosphorylate topoisomerase II, the formation of a stable topoisomerase II-casein kinase II association requires the presence of the beta subunit of the kinase. Both proteins engaged in this complex retain their catalytic activities. Naturally occurring polyamines and polyanionic compounds appear to be crucial factors governing the interaction between the two proteins. Although the biological significance of a stable catalytically active topoisomerase II-casein kinase II molecular complex remains to be defined, these observations suggest the possibility of a novel mechanism regulating topoisomerase II and casein kinase II activities.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)41614-6