Cloning and sequence analysis of the glucoamylase gene of Neurospora crassa

A 1.0-kb DNA fragment, corresponding to an internal region of the Neurospora crassa glucoamylase gene, gla-1, was generated from genomic DNA by the polymerase chain reaction, using oligonucleotide primers which had been deduced from the known N-terminal amino-acid sequence or from consensus regions...

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Bibliographic Details
Published in:Current genetics 1993-09, Vol.24 (3), p.205-211
Main Authors: Stone, P.J. (Leeds Univ. (United Kingdom). Dept. of Biochemistry and Molecular Biology), Makoff, A.J, Parish, J.H, Radford, A
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
Base Sequence
Biological and medical sciences
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
Cloning, Molecular
DNA, Fungal
Extracellular protein
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genes, Fungal
Genes. Genome
Glucan 1,4-alpha-Glucosidase - genetics
GLUCOAMILASA
GLUCOAMYLASE
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
NEUROSPORA
Neurospora crassa
Neurospora crassa - enzymology
Neurospora crassa - genetics
Polymerase Chain Reaction
Promoter Regions, Genetic
Protein Biosynthesis
Protein Precursors
Protein Sorting Signals
PROTEINAS
PROTEINE
SECUENCIA NUCLEICA
Sequence Homology, Amino Acid
SEQUENCE NUCLEIQUE
Signal sequence
Terminator Regions, Genetic
Transcription, Genetic
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Description
Summary:A 1.0-kb DNA fragment, corresponding to an internal region of the Neurospora crassa glucoamylase gene, gla-1, was generated from genomic DNA by the polymerase chain reaction, using oligonucleotide primers which had been deduced from the known N-terminal amino-acid sequence or from consensus regions within the aligned amino-acid sequences of other fungal glucoamylases. The fragment was used to screen an N. crassa genomic DNA library. One clone contained the gene together with flanking regions and its sequence was determined. The gene was found to code for a preprotein of 626 amino acids, 35 of which constitute a signal and propeptide region. The protein and the gene are compared with corresponding sequences in other fungi.
ISSN:0172-8083
1432-0983
DOI:10.1007/bf00351793