Urinary bladder smooth muscle regeneration utilizing bone marrow derived mesenchymal stem cell seeded elastomeric poly(1,8-octanediol-co-citrate) based thin films

Abstract Bladder regeneration studies have yielded inconclusive results possibly due to the use of unfavorable cells and primitive scaffold design. We hypothesized that human mesenchymal stem cells seeded onto poly(1,8-octanediol-co-citrate) elastomeric thin films would provide a suitable milieu for...

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Bibliographic Details
Published in:Biomaterials 2010-08, Vol.31 (24), p.6207-6217
Main Authors: Sharma, Arun K, Hota, Partha V, Matoka, Derek J, Fuller, Natalie J, Jandali, Danny, Thaker, Hatim, Ameer, Guillermo A, Cheng, Earl Y
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Azo Compounds
Bladder tissue engineering
Bone marrow
Bone Marrow Cells - cytology
Bone Marrow Cells - drug effects
Cell Survival - drug effects
Citrates - pharmacology
Citric Acid - pharmacology
Collagen - metabolism
Dentistry
Elastic Modulus - drug effects
Elastomer
Elastomers - pharmacology
Eosine Yellowish-(YS)
Female
Fluorescent Antibody Technique
Humans
Mesenchymal stem cell
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - drug effects
Methyl Green
Muscle, Smooth - cytology
Muscle, Smooth - drug effects
Muscle, Smooth - physiology
Polymers - pharmacology
Rats
Rats, Nude
Regeneration - drug effects
Regeneration - physiology
Smooth muscle cell
Staining and Labeling
Tissue Scaffolds - chemistry
Urinary Bladder - cytology
Urinary Bladder - drug effects
Urinary Bladder - physiology
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Summary:Abstract Bladder regeneration studies have yielded inconclusive results possibly due to the use of unfavorable cells and primitive scaffold design. We hypothesized that human mesenchymal stem cells seeded onto poly(1,8-octanediol-co-citrate) elastomeric thin films would provide a suitable milieu for partial bladder regeneration. POCfs were created by reacting citric acid with 1,8-octanediol and seeded on opposing faces with human MSCs and urothelial cells; normal bladder smooth muscle cells and UCs, or unseeded POCfs. Partial cystectomized nude rats were augmented with the aforementioned POCfs, enveloped with omentum and sacrificed at 4 and 10 weeks. Isolated bladders were subjected to Trichrome and anti-human γ-tubulin, calponin, caldesmon, smooth muscle γ-actin, and elastin stainings. Mechanical testing of POCfs revealed a Young’s modulus of 138 kPa with elongation 137% its initial length without permanent deformation demonstrating its high uniaxial elastic potential. Trichrome and immunofluorescent staining of MSC/UC POCf augmented bladders exhibited typical bladder architecture with muscle bundle formation and the expression and retention of bladder smooth muscle contractile proteins of human derivation. Quantitative morphometry of MSC/UC samples revealed muscle/collagen ratios approximately 1.75× greater than SMC/UC controls at 10 weeks. Data demonstrate MSC seeded POCfs support partial regeneration of bladder tissue in vivo.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2010.04.054