The immunoglobulin μ enhancer core establishes local factor access in nuclear chromatin independent of transcriptional stimulation

Factor access in chromatin has been proposed to be facilitated by transcriptional enhancers. With the aim of uncoupling factor access from transcriptional stimulation by protein-protein contacts, we analyzed the potential of enhancer fragments to confer accessibility upon a linked promoter for proka...

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Bibliographic Details
Published in:Genes & development 1993-10, Vol.7 (10), p.2016-2032
Main Authors: JENUWEIN, T, FORRESTER, W. C, RONG-GUO QIU, GROSSCHEDL, R
Format: Article
Language:English
Subjects:
Animals
B-Lymphocytes - metabolism
Base Sequence
Biological and medical sciences
Chromatin - metabolism
DNA Mutational Analysis
DNA-Directed RNA Polymerases - genetics
Enhancer Elements, Genetic - genetics
Fundamental and applied biological sciences. Psychology
Immunoglobulin mu-Chains - genetics
Mice
Mice, Transgenic
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
Viral Proteins
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Summary:Factor access in chromatin has been proposed to be facilitated by transcriptional enhancers. With the aim of uncoupling factor access from transcriptional stimulation by protein-protein contacts, we analyzed the potential of enhancer fragments to confer accessibility upon a linked promoter for prokaryotic T7 RNA polymerase. Access to the T7 promoter in pre-B cells from transgenic mice was examined by transcribing chromatin of isolated nuclei with T7 RNA polymerase. A 95-bp immunoglobulin mu enhancer core element was necessary and sufficient to confer accessibility upon the T7 promoter independent of its chromosomal position. This enhancer-dependent factor access could be uncoupled from an active transcriptional state of the transgene and was not accompanied by the formation of pronounced DNase I hypersensitive sites. Additional mu enhancer sequences comprising previously identified matrix attachment regions and a cryptic promoter were required to induce DNase I hypersensitivity. Together, these data provide evidence that the 95-bp mu enhancer core can establish localized factor access in nuclear chromatin independent of detectable transcription by endogenous polymerases and suggest that multiple steps are involved in the alteration of chromatin structure.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.7.10.2016