Endothelial cells enhance the human pokeweed mitogen lymphocyte response

The effect of endothelial cells (EC) on lymphocyte mitogen responses was examined. Irradiated or mitomycin C treated EC were co‐cultured with allogeneic peripheral blood mononuclear cells (PBM), and proliferative responses to pokeweed mitogen (PWM) and phytohemagglutinin (PHA) were assessed by 3H‐th...

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Bibliographic Details
Published in:Journal of leukocyte biology 1985-03, Vol.37 (3), p.305-317
Main Authors: W R Shanahan, Jr, D M Weston, J H Korn
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Biological and medical sciences
Cell Adhesion
Cell interactions
endothelial cells
Endothelium - cytology
Fetal Blood - cytology
Fibroblasts - physiology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunobiology
Indomethacin - pharmacology
Lymphocyte Activation
lymphocytes
pokeweed mitogen
Umbilical Cord - cytology
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Summary:The effect of endothelial cells (EC) on lymphocyte mitogen responses was examined. Irradiated or mitomycin C treated EC were co‐cultured with allogeneic peripheral blood mononuclear cells (PBM), and proliferative responses to pokeweed mitogen (PWM) and phytohemagglutinin (PHA) were assessed by 3H‐thymidine incorporation. Compared to lymphocyte responses in the absence of EC, EC co‐culture enhanced PWM responses at 72 hours by 55 ± 28%, 103 ± 24%, and 96 ± 9% at EC:PBM ratios of 1:30, 1:10, and 1:3, respectively. The EC co‐culture also resulted in signficant lymphocyte responses to otherwise submitogenic doses of PWM (10−4 μ g/ml) as well as an accelerated kinetics of response. There was no effect of EC on PHA responses. The EC effect appeared not to require cell contact for its expression; however, supernates of EC cultures were not capable of reproducing the effect. On a cell‐for‐cell basis, EC were more potent in enhancing responses of adherent‐cell‐depleted lymphocytes than either allogeneic or syngeneic monocytes. Fibroblasts could not substitute for EC in enhancing PWM response, suggesting that the effect was not a nonspecific feeder phenomenon. The EC may play a role in modulating some immune responses in vivo, especially those occurring in areas of inflammation, neovascularization, and endothelial cell proliferation.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.37.3.305