Molecular Analysis of VH and VL Regions Expressed in IgG-Bearing Chronic Lymphocytic Leukemia (CLL): Further Evidence That CLL Is a Heterogeneous Group of Tumors

We report the heavy (H) and light (L) chain variable (V) region sequences of cDNAs encoding the Ig receptor of two cases of CD5 + IgG-bearing CLL P87 and P103. In both CLL cases the H chain was encoded by members of the VH3 gene family. The L chain expressed by P87 belonged to the VλIV subgroup, whe...

Full description

Saved in:
Bibliographic Details
Published in:Blood 1993-09, Vol.82 (5), p.1626-1631
Main Authors: Ebeling, Saskia B., Schutte, Mieke E.M., Logtenberg, Ton
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Cloning, Molecular
Gene Expression
Hematologic and hematopoietic diseases
Humans
Immunoglobulin G - analysis
Immunoglobulin Variable Region - analysis
Immunoglobulin Variable Region - genetics
Leukemia, Lymphocytic, Chronic, B-Cell - genetics
Leukemia, Lymphocytic, Chronic, B-Cell - immunology
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Medical sciences
Molecular Sequence Data
Receptors, Antigen, B-Cell - analysis
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report the heavy (H) and light (L) chain variable (V) region sequences of cDNAs encoding the Ig receptor of two cases of CD5 + IgG-bearing CLL P87 and P103. In both CLL cases the H chain was encoded by members of the VH3 gene family. The L chain expressed by P87 belonged to the VλIV subgroup, whereas P103 used a member of the VϰIII subgroup. The VH3.P87 gene differed by only three nucleotides from 38P1, a VH3 gene previously cloned from a fetal liver cDNA library. Nucleotide sequence analysis demonstrated that the VϰIII.P103 gene differed by seven nucleotides from its most homologous germline counterpart, the Humkv325 gene, a highly conserved gene frequently expressed in IgM-bearing CLL. The nucleotide sequences of VH3.P103 and VϰIV.P87 could not be reliably matched with reported germline V genes. The analysis of multiple independently obtained VH and VL cDNA clones from each tumor showed a lack of intraclonal diversification. The data show that V regions expressed in isotype-switched CD5+ CLL may be either in/near germline configuration or somatically mutated. Furthermore, these tumors, like their IgM-bearing counterparts, do not seem to undergo intraclonal diversification.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V82.5.1626.1626