Establishment and characterization of a human mixed-lineage, T-lymphoid/myeloid cell line (USP-91)

We report the establishment of a novel cell line from a pediatric patient with recurrent non-Hodgkin's lymphoma. This cell line, termed USP-91, showed both T-lymphoid cell as well as myeloid (ie, nonlymphoid) cell characteristics using a comprehensive multiparameter approach. The initial growth...

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Bibliographic Details
Published in:Blood 1993-09, Vol.82 (6), p.1829-1837
Main Authors: UMIEL, T, RETENMIER, C. W, SIEGEL, S, OTA, S, SHIMADA, H, TRAN, T. W, PATTENGALE, P. K
Format: Article
Language:English
Subjects:
Animals
Antigens, CD - analysis
Biological and medical sciences
Biopsy
Cell Line
Child, Preschool
Clone Cells
Culture Techniques - methods
Gene Rearrangement
Gene Rearrangement, T-Lymphocyte
Genes, Immunoglobulin
Hematologic and hematopoietic diseases
Humans
Karyotyping
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Lymphoma, Non-Hodgkin - genetics
Lymphoma, Non-Hodgkin - immunology
Lymphoma, Non-Hodgkin - pathology
Lymphoma, Non-Hodgkin - ultrastructure
Lymphoma, T-Cell - genetics
Lymphoma, T-Cell - immunology
Lymphoma, T-Cell - pathology
Lymphoma, T-Cell - ultrastructure
Male
Medical sciences
Mice
Recurrence
Skin Neoplasms - genetics
Skin Neoplasms - immunology
Skin Neoplasms - pathology
Skin Neoplasms - ultrastructure
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Summary:We report the establishment of a novel cell line from a pediatric patient with recurrent non-Hodgkin's lymphoma. This cell line, termed USP-91, showed both T-lymphoid cell as well as myeloid (ie, nonlymphoid) cell characteristics using a comprehensive multiparameter approach. The initial growth of this cell line was dependent on the presence of the murine stromal cell line, 14F1.1. Subsequently, a phenotypically stable, stroma-independent cell line was established. Although the recurrent biopsy material and the derivative cell line, USP-91, were clonally-derived from T-lineage lymphoid cells, as evidenced by the same rearrangement of the T-cell receptor-beta locus, USP-91 coexpressed both the T-cell antigens CD7, CD3, and CD4, and the myeloid antigens CD13, CD33, CD11b, and CD34. The myeloid features of USP-91 were most consistent with monocytic differentiation as these cells expressed alpha-napthol acetate esterase, lysozyme, alpha-1-antitrypsin, alpha-1-antichymotrypsin, as well as the cell surface receptor for macrophage colony-stimulating factor. In addition, incubation in the presence of phorbol esters induced USP-91 to exhibit morphologic and functional properties of mature mononuclear phagocytes. The expression of this bilineage phenotype suggests that USP-91 represents the malignant transformation of a progenitor cell capable of either myelomonocytic or T-lymphoid differentiation.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V82.6.1829.1829