An experimental study of use of absorbable plate in combination with self-setting α-tricalcium phosphate for orthognathic surgery

Objective The purpose of this study was to histologically and immunohistochemically evaluate bone formation using both self-setting α-tricalcium phosphate (α-TCP; Biopex) and absorbable plate (Super Fixsorb-MX) in rabbit cranium bone. Study design Twelve adult male Japanese white rabbits (12-16 wk,...

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Published in:Oral surgery, oral medicine, oral pathology, oral radiology and endodontics oral medicine, oral pathology, oral radiology and endodontics, 2010-11, Vol.110 (5), p.560-569
Main Authors: Okabe, Katsuhiko, DDS, Ueki, Koichiro, DDS, PhD, Marukawa, Kohei, DDS, PhD, Mukozawa, Aya, DDS, Miyazaki, Mao, DDS, Nakagawa, Kiyomasa, DDS, PhD
Format: Article
Language:English
Subjects:
Absorbable Implants
Animals
Biocompatible Materials - therapeutic use
Biological and medical sciences
Bone Morphogenetic Protein 2 - analysis
Bone Plates
Bone Regeneration - drug effects
Bone Screws
Bone Substitutes - therapeutic use
Calcium Phosphates - therapeutic use
Cell Count
Chondroitin Sulfates - therapeutic use
Coloring Agents
Dentistry
Hydroxyapatites - therapeutic use
Immunohistochemistry
Male
Medical sciences
Models, Animal
Nasal Bone - pathology
Nasal Bone - surgery
Orthognathic Surgical Procedures - instrumentation
Orthognathic Surgical Procedures - methods
Osteoblasts - pathology
Osteoclasts - pathology
Osteogenesis - drug effects
Otorhinolaryngology. Stomatology
Rabbits
Succinates - therapeutic use
Surgery
Time Factors
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Summary:Objective The purpose of this study was to histologically and immunohistochemically evaluate bone formation using both self-setting α-tricalcium phosphate (α-TCP; Biopex) and absorbable plate (Super Fixsorb-MX) in rabbit cranium bone. Study design Twelve adult male Japanese white rabbits (12-16 wk, 2.5-3.0 kg) were used. The surgical defects were made in the nasal bone of a rabbit, and Biopex was implanted in the left side and no material in the right side. Two-hole absorbable plate and 2 screws (Super Fixsorb-MX) were fixed across the defect in each side. The rabbits were killed at 1, 4, 12, and 24 weeks after surgery, and formalin-fixed specimens were embedded in acrylic resin. The specimens were stained with hematoxylin and eosin. For immunohistochemical analysis, the specimens were treated with bone morphogenetic protein 2 (BMP-2) antibodies. Finally, these were evaluated microscopically. Results New bone formation was observed in the region of absorbable plate and nasal membrane after >4 weeks. The area of new bone with Biopex was significantly larger than that of the control side after 1, 4, and 12 weeks ( P < .05). The number of BMP-2–stained cells in the experimental side was significantly larger than in the control side after 4 and 12 weeks ( P < .05). Conclusion This study suggests that the use of absorbable plate (Super Fixsorb-MX) in combination with Biopex could be useful and that both of Super Fixsorb-MX and Biopex could provide adequate bone regeneration.
ISSN:1079-2104
1528-395X
DOI:10.1016/j.tripleo.2010.02.033