Serum IgG Reactivity with 41-, 31-, and 28-kDa Larval Proteins of Strongyloides stercoralis in Individuals with Strongyloidiasis

Proteins from a deoxycholate-soluble extract of Strongyloides stercoralis infective larvae were separated by SDS-PAGE, blotted onto nitrocellulose paper, and reacted with sera from individuals with confirmed S. stercoralis infections (n = 100), suspected S. stercoralis infections in whom no larvae c...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 1993-09, Vol.168 (3), p.784-787
Main Authors: Conway, D. J., Bailey, J. W., Lindo, J. F., Robinson, R. D., Bundy, D. A. P., Bianco, A. E.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Helminth - blood
Antigens, Helminth - immunology
Biological and medical sciences
Blotting, Western
Cross Reactions
Diseases caused by nematodes
Enzyme-Linked Immunosorbent Assay
Helminthic diseases
Humans
Immunoglobulin G - blood
Infectious diseases
Larva - immunology
Medical sciences
Parasitic diseases
Sensitivity and Specificity
Strongyloides stercoralis - immunology
Strongyloidiasis - diagnosis
Strongyloidosis
Tropical medicine
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Proteins from a deoxycholate-soluble extract of Strongyloides stercoralis infective larvae were separated by SDS-PAGE, blotted onto nitrocellulose paper, and reacted with sera from individuals with confirmed S. stercoralis infections (n = 100), suspected S. stercoralis infections in whom no larvae could be detected (n = 27), and other nematode infections (40 with Wuchereria bancrofti, 20 with Onchocerca volvulus, 20 with Necator americanus, and 20 with mixed Ascaris lumbricoides and Trichuris trichiura infections). Immunodominant proteins of ∼41, 31, and 28 kDa were recognized by IgG in 91%,88%, and 90%, respectively, of sera from those with confirmed strongyloidiasis; in 100%, 100%, and 93% of sera from those with suspected strongyloidiasis; and in 9%, 12%, and 14% of sera from those infected with other nematodes. IgG reactivity to each of these proteins was a more specific means of immunodiagnosis than the currently used indirect ELISA; the methods were equally sensitive.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/168.3.784