The Presence of P-glycoprotein in L1210 Cells Directly Induces Down-Regulation of Cell Surface Saccharide Targets of Concanavalin A

Overexpression of P-glycoprotein (P-gp), a plasma membrane drug transporter (ABCB1, a member of the ABC transporter family), is the most prevalent cause of multidrug resistance in cancer tissues. Lectin concanavalin A (ConA) induces massive cell death of L1210 leukemia cells (S). Cell sublines of L1...

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Published in:Anticancer research 2010-09, Vol.30 (9), p.3661-3668
Main Authors: SULOVA, Zdena, DITTE, Peter, BREIER, Albert, KURUCOVA, Tatiana, POLAKOVA, Eva, ROGOZANOVA, Kristina, GIBALOVA, Lenka, SERES, Mário, SKVARKOVA, Lucia, SEDLAK, Ján, PASTOREK, Jaromir
Format: Article
Language:English
Subjects:
Animals
ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism
Biological and medical sciences
Cell Line, Tumor
Cell Membrane - metabolism
Cell Separation
Concanavalin A - metabolism
Down-Regulation
Drug Resistance, Neoplasm
Electrophoresis, Polyacrylamide Gel
Flow Cytometry
Glycoproteins - metabolism
Humans
Immunoblotting
Medical sciences
Mice
Microscopy, Confocal
Transfection
Tumors
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Summary:Overexpression of P-glycoprotein (P-gp), a plasma membrane drug transporter (ABCB1, a member of the ABC transporter family), is the most prevalent cause of multidrug resistance in cancer tissues. Lectin concanavalin A (ConA) induces massive cell death of L1210 leukemia cells (S). Cell sublines of L1210 in which P-gp overexpression was induced by selection with vincristine (R) or by stable transfection with a plasmid encoding full-length human P-gp (T) were less sensitive to ConA. Both P-gp-positive cell lines exhibited typical P-gp-mediated multidrug resistance. Resistance of R and T cells to ConA was associated with lower binding of ConA as compared to S cells when analysed by the following methods: (i) SDS PAGE and electroblotting of proteins in the crude membrane fraction followed by detection with biotinylated ConA and avidin-peroxidase, and (ii) fluorescent cytometry or confocal microscopy of the intact cells with surfaces labeled by FITC-ConA. These data indicated that the presence of P-glycoprotein in L1210 cells independently of the mode of its expression induced down-regulation of cell surface saccharide targets of ConA. Therefore, this feature may be considered as a secondary cellular response to P-glycoprotein expression.
ISSN:0250-7005
1791-7530