Cloning of cDNA coding for dihydroflavonol-4-reductase (DFR) and characterization of dfr expression in the corollas of Gerbera hybrida var. Regina (Compositae)

We are approaching corolla differentiation in Compositae by studying the regulation of flavonoid pathway genes during inflorescence development in gerbera. We have cloned a dfr cDNA from a ray floret corolla cDNA library of Gerbera hybrida var. Regina by a PCR technique based on homologies found in...

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Bibliographic Details
Published in:Plant molecular biology 1993-05, Vol.22 (2), p.183-193
Main Authors: Helariutta, Y. (Helsinki Univ. (Finland). Inst. of Biotechnology), Elomaa, P, Kotilainen, M, Seppaenen, P, Teeri, T.H
Format: Article
Language:English
Subjects:
ADN
Alcohol Oxidoreductases - genetics
Amino Acid Sequence
Base Sequence
Biological and medical sciences
CLONACION
CLONAGE
CLONING
Cloning, Molecular
COROLA
COROLLA
COROLLE
DNA
ETAPAS DE DESARROLLO DE LA PLANTA
EXPRESION GENICA
EXPRESSION DES GENES
FLAVONOIDE
FLAVONOIDES
FLAVONOIDS
Flavonoids - metabolism
Fundamental and applied biological sciences. Psychology
GENE
GENE EXPRESSION
Gene Expression Regulation
GENES
Genes, Plant - genetics
GERBERA
Gerbera hybrida
In Situ Hybridization
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Plant Development
PLANT DEVELOPMENTAL STAGES
Plants - genetics
Plants, Genetically Modified - genetics
RNA, Messenger - genetics
Sequence Analysis, DNA
Sequence Homology, Amino Acid
STADE DE DEVELOPPEMENT VEGETAL
Time Factors
Tissue Distribution
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Summary:We are approaching corolla differentiation in Compositae by studying the regulation of flavonoid pathway genes during inflorescence development in gerbera. We have cloned a dfr cDNA from a ray floret corolla cDNA library of Gerbera hybrida var. Regina by a PCR technique based on homologies found in genes isolated from other plant species. The functionality of the clone was tested in vivo by complementing the dihydrokaempferol accumulating petunia mutant line RL01. By Southern blot analysis, G. hybrida var. Regina was shown to harbour a small family of dfr genes, one member of which was deduced to be mainly responsible for the DFR activity in corolla. Dfr expression in corolla correlates with the anthocyanin accumulation pattern: it is basipetally induced, epidermally specific and restricted to the ligular part of corolla. By comparing the dfr expression in different floret types during inflorescence development, we could see that dfr expression reflects developmental schemes of the outermost ray and trans florets, contrasted with that of the disc florets.
ISSN:0167-4412
1573-5028
DOI:10.1007/bf00014927