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Mitochondria from ejaculated human spermatozoa do not synthesize proteins
Sperm motility is dependent on mitochondrial ATP production that relies on the coordinated expression of the mitochondrial and nuclear genomes. It is generally accepted that mammalian ejaculated spermatozoa retain the ability to synthesize mtDNA-encoded proteins but not most of the nuclear ones. Thi...
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| Published in: | FEBS letters 2003-10, Vol.553 (1), p.205-208 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c5275-9a5c8025adaa4f88ae08b8d833f1fd8b2584ca442885088cc99efc32674924333 |
| container_end_page | 208 |
| container_issue | 1 |
| container_start_page | 205 |
| container_title | FEBS letters |
| container_volume | 553 |
| creator | Dı́ez-Sánchez, Carmen Ruiz-Pesini, Eduardo Montoya, Julio Pérez-Martos, Acisclo Enrı́quez, José Antonio López-Pérez, Manuel J |
| description | Sperm motility is dependent on mitochondrial ATP production that relies on the coordinated expression of the mitochondrial and nuclear genomes. It is generally accepted that mammalian ejaculated spermatozoa retain the ability to synthesize mtDNA-encoded proteins but not most of the nuclear ones. This implies an asynchronous regulation of the oxidative phosphorylation-related genes encoded by each genome. Trying to investigate this issue, we unexpectedly found that ejaculated human spermatozoa do not synthesize mtDNA-encoded proteins. Moreover, we estimated that the discrepancy between our observations and those published elsewhere was due to a chloramphenicol-sensitive protein synthesis attributed to mitochondria that instead corresponds to contaminating bacteria. |
| doi_str_mv | 10.1016/S0014-5793(03)01013-5 |
| format | article |
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| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 2003-10, Vol.553 (1), p.205-208 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75760254 |
| source | Wiley-Blackwell Read & Publish Collection; Elsevier ScienceDirect Journals |
| subjects | CAP, chloramphenicol Cell Line DMEM, Dulbecco's modified Eagle's medium Ejaculation - physiology EME, emetine Humans Male Methionine - metabolism Mitochondria - genetics Mitochondria - metabolism Mitochondrial DNA Mitochondrial Proteins - biosynthesis Mitochondrial Proteins - genetics Mitochondrion OXPHOS, oxidative phosphorylation Penicillin G - pharmacology Protein synthesis SDS–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis Sperm Motility Spermatogenesis Spermatozoa - cytology Spermatozoa - drug effects Spermatozoon |
| title | Mitochondria from ejaculated human spermatozoa do not synthesize proteins |
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