Mitochondria from ejaculated human spermatozoa do not synthesize proteins

Sperm motility is dependent on mitochondrial ATP production that relies on the coordinated expression of the mitochondrial and nuclear genomes. It is generally accepted that mammalian ejaculated spermatozoa retain the ability to synthesize mtDNA-encoded proteins but not most of the nuclear ones. Thi...

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Bibliographic Details
Published in:FEBS letters 2003-10, Vol.553 (1), p.205-208
Main Authors: Dı́ez-Sánchez, Carmen, Ruiz-Pesini, Eduardo, Montoya, Julio, Pérez-Martos, Acisclo, Enrı́quez, José Antonio, López-Pérez, Manuel J
Format: Article
Language:English
Subjects:
CAP, chloramphenicol
Cell Line
DMEM, Dulbecco's modified Eagle's medium
Ejaculation - physiology
EME, emetine
Humans
Male
Methionine - metabolism
Mitochondria - genetics
Mitochondria - metabolism
Mitochondrial DNA
Mitochondrial Proteins - biosynthesis
Mitochondrial Proteins - genetics
Mitochondrion
OXPHOS, oxidative phosphorylation
Penicillin G - pharmacology
Protein synthesis
SDS–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis
Sperm Motility
Spermatogenesis
Spermatozoa - cytology
Spermatozoa - drug effects
Spermatozoon
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Summary:Sperm motility is dependent on mitochondrial ATP production that relies on the coordinated expression of the mitochondrial and nuclear genomes. It is generally accepted that mammalian ejaculated spermatozoa retain the ability to synthesize mtDNA-encoded proteins but not most of the nuclear ones. This implies an asynchronous regulation of the oxidative phosphorylation-related genes encoded by each genome. Trying to investigate this issue, we unexpectedly found that ejaculated human spermatozoa do not synthesize mtDNA-encoded proteins. Moreover, we estimated that the discrepancy between our observations and those published elsewhere was due to a chloramphenicol-sensitive protein synthesis attributed to mitochondria that instead corresponds to contaminating bacteria.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(03)01013-5