Purification and Characterization of Three Members of the Photolyase/Cryptochrome Family Blue-light Photoreceptors from Vibrio cholerae

The sequence of Vibrio cholerae genome revealed three genes belonging to the photolyase/cryptochrome blue-light photoreceptor family. The proteins encoded by the three genes were purified and characterized. All three proteins contain folate and flavin cofactors and have absorption peaks in the range...

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Bibliographic Details
Published in:The Journal of biological chemistry 2003-10, Vol.278 (40), p.39143-39154
Main Authors: Worthington, Erin N., Kavakli, İ.Halil, Berrocal-Tito, Gloria, Bondo, Bruce E., Sancar, Aziz
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Cloning, Molecular
Cry1 gene
Cry1 toxin
Cry2 gene
Cry2 toxin
Cryptochromes
Deoxyribodipyrimidine Photo-Lyase - chemistry
Deoxyribodipyrimidine Photo-Lyase - isolation & purification
Dimerization
Dose-Response Relationship, Radiation
Drosophila Proteins
Electrophoresis, Polyacrylamide Gel
Escherichia coli - metabolism
Eye Proteins
flavin
Flavin-Adenine Dinucleotide - analogs & derivatives
Flavin-Adenine Dinucleotide - chemistry
Flavoproteins - chemistry
Flavoproteins - isolation & purification
Hot Temperature
Ligands
Light
Molecular Sequence Data
Mutation
photolyase
Photoreceptor Cells - chemistry
Photoreceptor Cells, Invertebrate
phr gene
phr protein
Plasmids - metabolism
Receptors, G-Protein-Coupled
RNA - metabolism
Sequence Homology, Amino Acid
Spectrometry, Fluorescence
Spectrophotometry
Ultraviolet Rays
Vibrio cholerae
Vibrio cholerae - metabolism
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Summary:The sequence of Vibrio cholerae genome revealed three genes belonging to the photolyase/cryptochrome blue-light photoreceptor family. The proteins encoded by the three genes were purified and characterized. All three proteins contain folate and flavin cofactors and have absorption peaks in the range of 350-500 nm. Only one of the three, VcPhr, is a photolyase specific for cyclobutane pyrimidine dimers. The other two are cryptochromes and were designated VcCry1 and VcCry2, respectively. Mutation of phr abolishes photoreactivation of UV-induced killing, whereas mutations in cry1 and cry2 do not affect photorepair activity. VcCry1 exhibits some unique features. Of all cryptochromes characterized to date, it is the only one that contains stoichiometric amounts of both chromophores and retains its flavin cofactor in the two-electron reduced FADH2 form. In addition, VcCry1 exhibits RNA binding activity and co-purifies with an RNA of 60-70 nucleotides in length.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M305792200