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The hemizona assay: its role in identifying male factor infertility in assisted reproduction
To identify male factor infertility among a group of patients in an assisted reproductive program (phase 1) and to evaluate the hemizona assay (HZA) in the diagnosis and prognosis of such a program (phase 2). The IVF performance of normal gametes in the Tygerberg program were critically evaluated. F...
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| Published in: | Fertility and sterility 1993-05, Vol.59 (5), p.1075-1080 |
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| container_title | Fertility and sterility |
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| creator | Franken, Daniel R. Acosta, Anibal A. Kruger, Thinus F. Lombard, Carl J. Oehninger, Sergio Hodgen, Gary D. |
| description | To identify male factor infertility among a group of patients in an assisted reproductive program (phase 1) and to evaluate the hemizona assay (HZA) in the diagnosis and prognosis of such a program (phase 2).
The IVF performance of normal gametes in the Tygerberg program were critically evaluated. Female patients were classified as pure tubal factor infertility, having a normal FSH:LH ratio on day 3 of the menstrual cycle. All participating women produced three or more preovulatory oocytes at retrieval and were inseminated with sperm considered normal by all present diagnostic criteria. The total and normal fertilization rate thresholds were defined in that group. Using those thresholds, couples tested for sperm binding in the HZA (n=48) were used and divided into two groups according to their fertilization rates, namely group 1, low fertilization (55%).
University-based tertiary care center.
Ninety-nine couples (589 oocytes) with pure tubal factor infertility and normal male factor were used in phase 1. Forty-eight couples with normal and abnormal male factors that had both HZA performed and IVF treatment were included in phase 2.
Investigation of the performance of normal gametes in 99 couples (589 oocytes) revealed the total fertilization rate (total number of oocytes fertilized/total number of oocytes inseminated) was (mean±SD) 88.6%±16.8% and the normal fertilization rate (total number of oocytes with normal fertilization/total number of oocytes inseminated) was 81.3%±22%. The minimum total fertilization rate that can be considered normal in the Tygerberg program using mean−2 SD is therefore 55% and for normal fertilization rate is 37%. The group with low fertilization rate ( |
| doi_str_mv | 10.1016/S0015-0282(16)55931-7 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75719640</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0015028216559317</els_id><sourcerecordid>75719640</sourcerecordid><originalsourceid>FETCH-LOGICAL-c389t-7a9ffcedb5a47b02ae883943ea33ff5d331f5fa4ba17a2742122a21a3f3db173</originalsourceid><addsrcrecordid>eNqFkElvFDEQRi0ECpOBnxCpDwiRQyde291cIhSxRIqUA3NEsqrdZVKol2B7kIZfjyczmisnL9-rKvsxdiH4leCiuf7OuTA1l638IJpLYzolavuCrYQxTW0ao16y1Ql5zc5T-sU5b4SVZ-ys1W3Z2RX7sXnE6hEn-rvMUEFKsPtYUU5VXEasaK5owDlT2NH8s5qg3AXweYklChgzjZR3e6xUUso4VBGf4jJsfaZlfsNeBRgTvj2ua7b58nlz-62-f_h6d_vpvvaq7XJtoQvB49Ab0LbnErBtVacVglIhmEEpEUwA3YOwIK2WQkqQAlRQQy-sWrP3h7Zl8u8tpuwmSh7HEWZctslZY0XXaF5AcwB9XFKKGNxTpAnizgnu9lLds1S3N-bK6Vmq2w-4OA7Y9hMOp6qjxZK_O-aQPIwhwuwpnTBttdGl1ZrdHDAsLv4QRpc84Vx-ThF9dsNC_3nIP6x7lNE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>75719640</pqid></control><display><type>article</type><title>The hemizona assay: its role in identifying male factor infertility in assisted reproduction</title><source>ScienceDirect®</source><creator>Franken, Daniel R. ; Acosta, Anibal A. ; Kruger, Thinus F. ; Lombard, Carl J. ; Oehninger, Sergio ; Hodgen, Gary D.</creator><creatorcontrib>Franken, Daniel R. ; Acosta, Anibal A. ; Kruger, Thinus F. ; Lombard, Carl J. ; Oehninger, Sergio ; Hodgen, Gary D.</creatorcontrib><description>To identify male factor infertility among a group of patients in an assisted reproductive program (phase 1) and to evaluate the hemizona assay (HZA) in the diagnosis and prognosis of such a program (phase 2).
The IVF performance of normal gametes in the Tygerberg program were critically evaluated. Female patients were classified as pure tubal factor infertility, having a normal FSH:LH ratio on day 3 of the menstrual cycle. All participating women produced three or more preovulatory oocytes at retrieval and were inseminated with sperm considered normal by all present diagnostic criteria. The total and normal fertilization rate thresholds were defined in that group. Using those thresholds, couples tested for sperm binding in the HZA (n=48) were used and divided into two groups according to their fertilization rates, namely group 1, low fertilization (<55%) and group 2, normal fertilization (>55%).
University-based tertiary care center.
Ninety-nine couples (589 oocytes) with pure tubal factor infertility and normal male factor were used in phase 1. Forty-eight couples with normal and abnormal male factors that had both HZA performed and IVF treatment were included in phase 2.
Investigation of the performance of normal gametes in 99 couples (589 oocytes) revealed the total fertilization rate (total number of oocytes fertilized/total number of oocytes inseminated) was (mean±SD) 88.6%±16.8% and the normal fertilization rate (total number of oocytes with normal fertilization/total number of oocytes inseminated) was 81.3%±22%. The minimum total fertilization rate that can be considered normal in the Tygerberg program using mean−2 SD is therefore 55% and for normal fertilization rate is 37%. The group with low fertilization rate (<55%) showed a mean hemizona index (HZI) significantly lower; nevertheless, the distribution overlapping indicates a low discriminating power of the HZA. A sensitivity of 75% and a specificity of 75% were found; the positive and negative predictive values were 81% and 68%, respectively.
The results indicated the HZA and HZI contribute important information and can serve in conjunction with other semen characteristics as useful tools during the diagnosis of the male factor in assisted reproduction.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(16)55931-7</identifier><identifier>PMID: 8486177</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Biological and medical sciences ; Birth control ; Chorionic Gonadotropin - therapeutic use ; Clomiphene - therapeutic use ; Discriminant Analysis ; Female ; Fertilization in Vitro ; Follicle Stimulating Hormone - blood ; Gynecology. Andrology. Obstetrics ; Hemizona assay ; Humans ; Infertility, Male - diagnosis ; Infertility, Male - physiopathology ; Luteinizing Hormone - blood ; Male ; male factor infertility ; Medical sciences ; Menotropins - therapeutic use ; Menstrual Cycle ; sperm binding ; Sperm Motility ; Sperm-Ovum Interactions ; Spermatozoa - cytology ; Spermatozoa - physiology ; Sterility. Assisted procreation</subject><ispartof>Fertility and sterility, 1993-05, Vol.59 (5), p.1075-1080</ispartof><rights>1993 American Society for Reproductive Medicine</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-7a9ffcedb5a47b02ae883943ea33ff5d331f5fa4ba17a2742122a21a3f3db173</citedby><cites>FETCH-LOGICAL-c389t-7a9ffcedb5a47b02ae883943ea33ff5d331f5fa4ba17a2742122a21a3f3db173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0015028216559317$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,786,790,3568,27957,27958,45815</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4745459$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8486177$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Franken, Daniel R.</creatorcontrib><creatorcontrib>Acosta, Anibal A.</creatorcontrib><creatorcontrib>Kruger, Thinus F.</creatorcontrib><creatorcontrib>Lombard, Carl J.</creatorcontrib><creatorcontrib>Oehninger, Sergio</creatorcontrib><creatorcontrib>Hodgen, Gary D.</creatorcontrib><title>The hemizona assay: its role in identifying male factor infertility in assisted reproduction</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>To identify male factor infertility among a group of patients in an assisted reproductive program (phase 1) and to evaluate the hemizona assay (HZA) in the diagnosis and prognosis of such a program (phase 2).
The IVF performance of normal gametes in the Tygerberg program were critically evaluated. Female patients were classified as pure tubal factor infertility, having a normal FSH:LH ratio on day 3 of the menstrual cycle. All participating women produced three or more preovulatory oocytes at retrieval and were inseminated with sperm considered normal by all present diagnostic criteria. The total and normal fertilization rate thresholds were defined in that group. Using those thresholds, couples tested for sperm binding in the HZA (n=48) were used and divided into two groups according to their fertilization rates, namely group 1, low fertilization (<55%) and group 2, normal fertilization (>55%).
University-based tertiary care center.
Ninety-nine couples (589 oocytes) with pure tubal factor infertility and normal male factor were used in phase 1. Forty-eight couples with normal and abnormal male factors that had both HZA performed and IVF treatment were included in phase 2.
Investigation of the performance of normal gametes in 99 couples (589 oocytes) revealed the total fertilization rate (total number of oocytes fertilized/total number of oocytes inseminated) was (mean±SD) 88.6%±16.8% and the normal fertilization rate (total number of oocytes with normal fertilization/total number of oocytes inseminated) was 81.3%±22%. The minimum total fertilization rate that can be considered normal in the Tygerberg program using mean−2 SD is therefore 55% and for normal fertilization rate is 37%. The group with low fertilization rate (<55%) showed a mean hemizona index (HZI) significantly lower; nevertheless, the distribution overlapping indicates a low discriminating power of the HZA. A sensitivity of 75% and a specificity of 75% were found; the positive and negative predictive values were 81% and 68%, respectively.
The results indicated the HZA and HZI contribute important information and can serve in conjunction with other semen characteristics as useful tools during the diagnosis of the male factor in assisted reproduction.</description><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Chorionic Gonadotropin - therapeutic use</subject><subject>Clomiphene - therapeutic use</subject><subject>Discriminant Analysis</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Follicle Stimulating Hormone - blood</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Hemizona assay</subject><subject>Humans</subject><subject>Infertility, Male - diagnosis</subject><subject>Infertility, Male - physiopathology</subject><subject>Luteinizing Hormone - blood</subject><subject>Male</subject><subject>male factor infertility</subject><subject>Medical sciences</subject><subject>Menotropins - therapeutic use</subject><subject>Menstrual Cycle</subject><subject>sperm binding</subject><subject>Sperm Motility</subject><subject>Sperm-Ovum Interactions</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - physiology</subject><subject>Sterility. Assisted procreation</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNqFkElvFDEQRi0ECpOBnxCpDwiRQyde291cIhSxRIqUA3NEsqrdZVKol2B7kIZfjyczmisnL9-rKvsxdiH4leCiuf7OuTA1l638IJpLYzolavuCrYQxTW0ao16y1Ql5zc5T-sU5b4SVZ-ys1W3Z2RX7sXnE6hEn-rvMUEFKsPtYUU5VXEasaK5owDlT2NH8s5qg3AXweYklChgzjZR3e6xUUso4VBGf4jJsfaZlfsNeBRgTvj2ua7b58nlz-62-f_h6d_vpvvaq7XJtoQvB49Ab0LbnErBtVacVglIhmEEpEUwA3YOwIK2WQkqQAlRQQy-sWrP3h7Zl8u8tpuwmSh7HEWZctslZY0XXaF5AcwB9XFKKGNxTpAnizgnu9lLds1S3N-bK6Vmq2w-4OA7Y9hMOp6qjxZK_O-aQPIwhwuwpnTBttdGl1ZrdHDAsLv4QRpc84Vx-ThF9dsNC_3nIP6x7lNE</recordid><startdate>19930501</startdate><enddate>19930501</enddate><creator>Franken, Daniel R.</creator><creator>Acosta, Anibal A.</creator><creator>Kruger, Thinus F.</creator><creator>Lombard, Carl J.</creator><creator>Oehninger, Sergio</creator><creator>Hodgen, Gary D.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930501</creationdate><title>The hemizona assay: its role in identifying male factor infertility in assisted reproduction</title><author>Franken, Daniel R. ; Acosta, Anibal A. ; Kruger, Thinus F. ; Lombard, Carl J. ; Oehninger, Sergio ; Hodgen, Gary D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-7a9ffcedb5a47b02ae883943ea33ff5d331f5fa4ba17a2742122a21a3f3db173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Chorionic Gonadotropin - therapeutic use</topic><topic>Clomiphene - therapeutic use</topic><topic>Discriminant Analysis</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Follicle Stimulating Hormone - blood</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Hemizona assay</topic><topic>Humans</topic><topic>Infertility, Male - diagnosis</topic><topic>Infertility, Male - physiopathology</topic><topic>Luteinizing Hormone - blood</topic><topic>Male</topic><topic>male factor infertility</topic><topic>Medical sciences</topic><topic>Menotropins - therapeutic use</topic><topic>Menstrual Cycle</topic><topic>sperm binding</topic><topic>Sperm Motility</topic><topic>Sperm-Ovum Interactions</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - physiology</topic><topic>Sterility. Assisted procreation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Franken, Daniel R.</creatorcontrib><creatorcontrib>Acosta, Anibal A.</creatorcontrib><creatorcontrib>Kruger, Thinus F.</creatorcontrib><creatorcontrib>Lombard, Carl J.</creatorcontrib><creatorcontrib>Oehninger, Sergio</creatorcontrib><creatorcontrib>Hodgen, Gary D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Franken, Daniel R.</au><au>Acosta, Anibal A.</au><au>Kruger, Thinus F.</au><au>Lombard, Carl J.</au><au>Oehninger, Sergio</au><au>Hodgen, Gary D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The hemizona assay: its role in identifying male factor infertility in assisted reproduction</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>1993-05-01</date><risdate>1993</risdate><volume>59</volume><issue>5</issue><spage>1075</spage><epage>1080</epage><pages>1075-1080</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>To identify male factor infertility among a group of patients in an assisted reproductive program (phase 1) and to evaluate the hemizona assay (HZA) in the diagnosis and prognosis of such a program (phase 2).
The IVF performance of normal gametes in the Tygerberg program were critically evaluated. Female patients were classified as pure tubal factor infertility, having a normal FSH:LH ratio on day 3 of the menstrual cycle. All participating women produced three or more preovulatory oocytes at retrieval and were inseminated with sperm considered normal by all present diagnostic criteria. The total and normal fertilization rate thresholds were defined in that group. Using those thresholds, couples tested for sperm binding in the HZA (n=48) were used and divided into two groups according to their fertilization rates, namely group 1, low fertilization (<55%) and group 2, normal fertilization (>55%).
University-based tertiary care center.
Ninety-nine couples (589 oocytes) with pure tubal factor infertility and normal male factor were used in phase 1. Forty-eight couples with normal and abnormal male factors that had both HZA performed and IVF treatment were included in phase 2.
Investigation of the performance of normal gametes in 99 couples (589 oocytes) revealed the total fertilization rate (total number of oocytes fertilized/total number of oocytes inseminated) was (mean±SD) 88.6%±16.8% and the normal fertilization rate (total number of oocytes with normal fertilization/total number of oocytes inseminated) was 81.3%±22%. The minimum total fertilization rate that can be considered normal in the Tygerberg program using mean−2 SD is therefore 55% and for normal fertilization rate is 37%. The group with low fertilization rate (<55%) showed a mean hemizona index (HZI) significantly lower; nevertheless, the distribution overlapping indicates a low discriminating power of the HZA. A sensitivity of 75% and a specificity of 75% were found; the positive and negative predictive values were 81% and 68%, respectively.
The results indicated the HZA and HZI contribute important information and can serve in conjunction with other semen characteristics as useful tools during the diagnosis of the male factor in assisted reproduction.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>8486177</pmid><doi>10.1016/S0015-0282(16)55931-7</doi><tpages>6</tpages></addata></record> |
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| ispartof | Fertility and sterility, 1993-05, Vol.59 (5), p.1075-1080 |
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| source | ScienceDirect® |
| subjects | Biological and medical sciences Birth control Chorionic Gonadotropin - therapeutic use Clomiphene - therapeutic use Discriminant Analysis Female Fertilization in Vitro Follicle Stimulating Hormone - blood Gynecology. Andrology. Obstetrics Hemizona assay Humans Infertility, Male - diagnosis Infertility, Male - physiopathology Luteinizing Hormone - blood Male male factor infertility Medical sciences Menotropins - therapeutic use Menstrual Cycle sperm binding Sperm Motility Sperm-Ovum Interactions Spermatozoa - cytology Spermatozoa - physiology Sterility. Assisted procreation |
| title | The hemizona assay: its role in identifying male factor infertility in assisted reproduction |
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