The hemizona assay: its role in identifying male factor infertility in assisted reproduction

To identify male factor infertility among a group of patients in an assisted reproductive program (phase 1) and to evaluate the hemizona assay (HZA) in the diagnosis and prognosis of such a program (phase 2). The IVF performance of normal gametes in the Tygerberg program were critically evaluated. F...

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Bibliographic Details
Published in:Fertility and sterility 1993-05, Vol.59 (5), p.1075-1080
Main Authors: Franken, Daniel R., Acosta, Anibal A., Kruger, Thinus F., Lombard, Carl J., Oehninger, Sergio, Hodgen, Gary D.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Birth control
Chorionic Gonadotropin - therapeutic use
Clomiphene - therapeutic use
Discriminant Analysis
Female
Fertilization in Vitro
Follicle Stimulating Hormone - blood
Gynecology. Andrology. Obstetrics
Hemizona assay
Humans
Infertility, Male - diagnosis
Infertility, Male - physiopathology
Luteinizing Hormone - blood
Male
male factor infertility
Medical sciences
Menotropins - therapeutic use
Menstrual Cycle
sperm binding
Sperm Motility
Sperm-Ovum Interactions
Spermatozoa - cytology
Spermatozoa - physiology
Sterility. Assisted procreation
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Summary:To identify male factor infertility among a group of patients in an assisted reproductive program (phase 1) and to evaluate the hemizona assay (HZA) in the diagnosis and prognosis of such a program (phase 2). The IVF performance of normal gametes in the Tygerberg program were critically evaluated. Female patients were classified as pure tubal factor infertility, having a normal FSH:LH ratio on day 3 of the menstrual cycle. All participating women produced three or more preovulatory oocytes at retrieval and were inseminated with sperm considered normal by all present diagnostic criteria. The total and normal fertilization rate thresholds were defined in that group. Using those thresholds, couples tested for sperm binding in the HZA (n=48) were used and divided into two groups according to their fertilization rates, namely group 1, low fertilization (55%). University-based tertiary care center. Ninety-nine couples (589 oocytes) with pure tubal factor infertility and normal male factor were used in phase 1. Forty-eight couples with normal and abnormal male factors that had both HZA performed and IVF treatment were included in phase 2. Investigation of the performance of normal gametes in 99 couples (589 oocytes) revealed the total fertilization rate (total number of oocytes fertilized/total number of oocytes inseminated) was (mean±SD) 88.6%±16.8% and the normal fertilization rate (total number of oocytes with normal fertilization/total number of oocytes inseminated) was 81.3%±22%. The minimum total fertilization rate that can be considered normal in the Tygerberg program using mean−2 SD is therefore 55% and for normal fertilization rate is 37%. The group with low fertilization rate (
ISSN:0015-0282
1556-5653
DOI:10.1016/S0015-0282(16)55931-7