An osmosensing signal transduction pathway in yeast

Yeast genes were isolated that are required for restoring the osmotic gradient across the cell membrane in response to increased external osmolarity. Two of these genes, HOG1 and PBS2, encode members of the mitogen-activated protein kinase (MAP kinase) and MAP kinase gene families, respectively. MAP...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1993-03, Vol.259 (5102), p.1760-1763
Main Authors: Brewster, J.L. (Rice University, Houston, TX), De Valoir, T, Dwyer, N.D, Winter, E, Gustin, M.C
Format: Article
Language:English
Subjects:
Action of physical and chemical agents
Amino Acid Sequence
Amino acids
Animal cells
Biological and medical sciences
Blotting, Northern
Calcium-Calmodulin-Dependent Protein Kinases
Cell growth
Cell membranes
Cellular biology
Coding
Cytology
DNA
Feedback (Response)
Fluid-electrolyte balance
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genes, Fungal
Genetics
Genomics
Microbiology
Molecular Sequence Data
Mycology
Osmolar Concentration
Osmoregulation
Osmosis
Phosphorylation
Phosphothreonine - metabolism
Phosphotyrosine
Plasmids
PRESION OSMOTICA
PRESSION OSMOTIQUE
Protein Kinases - chemistry
Protein Kinases - genetics
PROTEINA QUINASA
PROTEINE KINASE
Restriction Mapping
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
Signal Transduction - genetics
Tyrosine - analogs & derivatives
Tyrosine - metabolism
Water-electrolyte balance (Physiology)
Water-Electrolyte Balance - genetics
Yeast
Yeast (Food product)
Yeasts
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Summary:Yeast genes were isolated that are required for restoring the osmotic gradient across the cell membrane in response to increased external osmolarity. Two of these genes, HOG1 and PBS2, encode members of the mitogen-activated protein kinase (MAP kinase) and MAP kinase gene families, respectively. MAP kinases are activated by extracellular ligands such as growth factors and function as intermediate kinases in protein phosphorylation cascades. A rapid, PBS2-dependent tyrosine phosphorylation of HOG1 protein occurred in response to increases in extracellular osmolarity. These data define a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment
ISSN:0036-8075
1095-9203
DOI:10.1126/science.7681220