Macrophage phenotype regulation by colony-stimulating factors at bone marrow level

Macrophages (mφs) can be divided into several subpopulations, which differ in phenotype, function, and localization patterns. However, little is known about the mechanisms that regulate this heterogeneity. We investigated whether mφ heterogeneity is regulated by colony‐stimulating factors (CSFs) at...

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Bibliographic Details
Published in:Journal of leukocyte biology 1993-03, Vol.53 (3), p.249-255
Main Authors: Wijffels, J.F.A.M., Rover, Z., Kraal, G., Beelen, R.H.J.
Format: Article
Language:English
Subjects:
Animals
Antigen-Presenting Cells - physiology
Biological and medical sciences
Bone Marrow Cells
Cerebrospinal Fluid - physiology
Cloning, Molecular
colony‐stimulating factor 1
Culture Media, Conditioned
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
granulocyte‐macrophage colony‐stimulating factor
Immunobiology
macrophage precursor cells
Macrophages - immunology
Macrophages - physiology
Mice
Mice, Inbred C57BL
Monocytes, macrophages
multi‐CSF
Myeloid cells: ontogeny, maturation, markers, receptors
Phenotype
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Summary:Macrophages (mφs) can be divided into several subpopulations, which differ in phenotype, function, and localization patterns. However, little is known about the mechanisms that regulate this heterogeneity. We investigated whether mφ heterogeneity is regulated by colony‐stimulating factors (CSFs) at the bone marrow level. By clonal expansion of bone marrow‐derived precursor cells in die presence of CSF‐1, granulocyte‐macrophage CSF or multi‐CSF (interleukin‐3), phenotypic heterogeneity was observed between mφ colonies. Heterogeneity was found especially when different CSF culture conditions were used but also between mφ colonies derived under the same CSF culture condition. Our results illustrate that CSFs from the bone marrow hemopoietic microenvironment are important for the induction of phenotypic heterogeneity within the progeny of cloned mφ precursor cells during maturation and differentiation in vitro.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.53.3.249