Human gastric mucin: identification of a unique species by expression cloning

Gastric mucin is a large glycoprotein which is thought to play a major role in the protection of the gastrointestinal tract from acid, proteases, pathogenic microorganisms, and mechanical trauma. In this paper we describe the isolation by expression cloning and characterization of cDNAs which code f...

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Bibliographic Details
Published in:The Journal of biological chemistry 1993-03, Vol.268 (8), p.5879-5885
Main Authors: Toribara, N.W, Roberton, A.M, Ho, S.B, Kuo, W.L, Gum, E, Hicks, J.W, Gum, J.R. Jr, Byrd, J.C, Siddiki, B, Kim, Y.S
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Base Sequence
Biological and medical sciences
cDNA
CHIMIE
Chromosome Mapping
Chromosomes, Human, Pair 11
Cloning, Molecular
DNA - isolation & purification
ESTOMAC
ESTOMAGO
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
Gastric Mucins - analysis
Gastric Mucins - genetics
gastric tissue
GENE
GENERO HUMANO
GENES
GENRE HUMAIN
Glycoproteins
Humans
Immunoenzyme Techniques
IMMUNOLOGIE
INMUNOLOGIA
LOCALISATION DE GENE
LOCALIZACION DE GENES
man
MEMBRANA MUCOSA
Molecular Sequence Data
mucin
MUCINA
MUCINE
Mucins - analysis
Mucins - genetics
MUQUEUSE
nucleotide sequence
prediction
Proteins
QUIMICA
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
Stomach - chemistry
VESICULA BILIAR
VESICULE BILIAIRE
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Summary:Gastric mucin is a large glycoprotein which is thought to play a major role in the protection of the gastrointestinal tract from acid, proteases, pathogenic microorganisms, and mechanical trauma. In this paper we describe the isolation by expression cloning and characterization of cDNAs which code for human gastric mucin. The cDNA sequence is characterized by a tandem repeat region whose individual repeat unit is 507 base pairs (169 amino acids) long. The translated sequence is rich in threonine, serine, and proline (31, 18, and 15%, respectively) and contains a relatively large amount of histidine (7.1%) and alanine (5.6%). RNA blot analysis shows a polydisperse pattern which is characteristic of mucins. Expression of this gene is highest in the stomach and gall bladder, with weaker expression in the terminal ileum and right colon. This expression pattern is different from other human mucins and indicates that this gene codes for a unique mucin. Fluorescence in situ hybridization techniques have localized this gene to chromosome 11p15.4-11p15.5. This is the third mucin to be localized to the 11p15 region and suggests a clustering of secretory mucin genes. We propose that this gene for human gastric mucin be called MUC6
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)53402-5