Eosinophil Migration in Atopic Dermatitis I: Increased Migratory Responses to N-Formyl-Methionyl-Leucyl-Phenylalanine, Neutrophil-Activating Factor, Platelet-Activating Factor, and Platelet Factor 4

Eosinophil granular protein deposits have been demonstrated in lesional atopic dermatitis skin. This suggests active tissue infiltration of eosinophils. To find an explanation for the tissue influx of eosinophils, eosinophil migration was studied in vitro by means of a microchemotaxis assay. Eosinop...

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Bibliographic Details
Published in:Journal of investigative dermatology 1993-02, Vol.100 (2), p.137-142
Main Authors: Bruijnzeel, Piet L.B., Kuijper, Philip H.M., Rihs, Silvia, Betz, Suzanne, Warringa, Ruud A.J., Koenderman, Leo
Format: Article
Language:English
Subjects:
Adolescent
Adult
Allergic diseases
Biological and medical sciences
Cell Movement - drug effects
Chemotaxis, Leukocyte - physiology
Complement C5a - pharmacology
Dermatitis, Atopic - blood
Dermatitis, Atopic - physiopathology
Eosinophils - physiology
Female
Humans
Immunopathology
Interleukin-5 - pharmacology
Interleukin-8 - pharmacology
Male
Medical sciences
Middle Aged
N-Formylmethionine Leucyl-Phenylalanine - pharmacology
Platelet Activating Factor - pharmacology
Platelet Factor 4 - pharmacology
Skin allergic diseases. Stinging insect allergies
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Summary:Eosinophil granular protein deposits have been demonstrated in lesional atopic dermatitis skin. This suggests active tissue infiltration of eosinophils. To find an explanation for the tissue influx of eosinophils, eosinophil migration was studied in vitro by means of a microchemotaxis assay. Eosinophils from the circulation of patients with atopic dermatitis in vitro compared with eosinophils from healthy donors. Eosinophils from patients with atopic dermatitis had significantly increased migratory responses toward close ranges of N-formyl-methionyl-lecucyl phenylalanine, neutrophil-activating factor, platelet-activating factor, and platelet factor 4. Eosinophils from normal individuals did not respond to N-formyl-methionyl-leucyl-phenylalanine and neutrophil-activating factor and responded only slightly to platelet tactor 4. The migratory responses toward tumor necrosis factor-α and complement factor C5a were identical in both groups. Interleukin-5, an eosinophil-selective cytokine, is a strong modulator of the migratory responses to these chemotaxins in eosinophils from normal donors. A migratory response toward N-formyl-methionyl-leucyl-phenylalanine and neutrophil-activating factor Was induced by interleukin-5, whereas the migratory response toward platelet-activating factor and platelet factor 4 was markedly potentiated. In contrast, the response to complement fragment C5a was only slightly influenced. Our findings indicate that the increased migratory responsiveness of eosinophils from patients with at0pic dermatitis to various chemotaxins reflects in vivo “priming” of eosinophils, presumably by circulating cytokines such as interleukm-5. This in vivo “priming” is not optimal because it can be further potentiated by renewed contact with interleukin-5.
ISSN:0022-202X
1523-1747
DOI:10.1111/1523-1747.ep12462781