Integration of a Fluorescent Molecular Biosensor into Self-Assembled Protein Nanowires: A Large Sensitivity Enhancement

Wire to wire: The hybrid Sup351‐61‐E2GFP‐MPH, which consists of the pH‐sensitive green fluorescent protein E2GFP, the enzyme methyl parathion hydrolase (MPH), and the yeast prion protein Sup351‐61 was obtained by gene fusion. A self‐assembled nanowire was formed through aggregation of the Sup351‐61...

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Published in:Angewandte Chemie (International ed.) 2010-09, Vol.49 (40), p.7243-7246
Main Authors: Leng, Yan, Wei, Hong-Ping, Zhang, Zhi-Ping, Zhou, Ya-Feng, Deng, Jiao-Yu, Cui, Zong-Qiang, Men, Dong, You, Xiang-Yu, Yu, Zi-Niu, Luo, Ming, Zhang, Xian-En
Format: Article
Language:English
Subjects:
Biosensing Techniques
biosensors
enzyme catalysis
Fluorescent Dyes - chemistry
fluorescent probes
Models, Molecular
nanowires
Nanowires - chemistry
self-assembly
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Summary:Wire to wire: The hybrid Sup351‐61‐E2GFP‐MPH, which consists of the pH‐sensitive green fluorescent protein E2GFP, the enzyme methyl parathion hydrolase (MPH), and the yeast prion protein Sup351‐61 was obtained by gene fusion. A self‐assembled nanowire was formed through aggregation of the Sup351‐61 subunit (see picture). The wire acts as a biosensor that can detect methyl parathion with a sensitivity 10 000 times greater than free E2GFP‐MPH.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201002452