Preventive effects of chebulic acid isolated from Terminalia chebula on advanced glycation endproduct-induced endothelial cell dysfunction

The aqueous extract of Terminalia chebular fruits was reported to have anti-hyperglycemia and anti-diabetic complication effects. The present study therefore investigated the protective mechanism of chebulic acid, a phenolcarboxylic acid compound isolated from the ripe fruits of Terminalia chebula a...

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Bibliographic Details
Published in:Journal of ethnopharmacology 2010-10, Vol.131 (3), p.567-574
Main Authors: Lee, Hyun-Sun, Koo, Yoon-Chang, Suh, Hyung Joo, Kim, Kyung-Yong, Lee, Kwang-Won
Format: Article
Language:English
Subjects:
advanced glycation endpoints
Advanced glycation endproducts
Biological and medical sciences
carboxylic acids
cell adhesion
Cell Adhesion - drug effects
Cell Line
Cells, Cultured
Chebulic acid
Diabetic complications
dosage
Dose-Response Relationship, Drug
endothelial cells
Endothelial Cells - drug effects
Endothelial Cells - metabolism
Endothelial dysfunction
Endothelium, Vascular - drug effects
Endothelium, Vascular - metabolism
Fruit
fruits (plant anatomy)
General pharmacology
Glycation End Products, Advanced - metabolism
glyceraldehyde
Glyceraldehyde - administration & dosage
human cell lines
Humans
Hydrolyzable Tannins - administration & dosage
Hydrolyzable Tannins - isolation & purification
Hydrolyzable Tannins - pharmacology
lymphocytes
Medical sciences
medicinal plants
monocytes
Monocytes - drug effects
Monocytes - metabolism
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
plant extracts
protective effect
reactive oxygen species
Reactive Oxygen Species - metabolism
Terminaila chebula
Terminalia - chemistry
Terminalia chebula
umbilical veins
Umbilical Veins - drug effects
Umbilical Veins - metabolism
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Summary:The aqueous extract of Terminalia chebular fruits was reported to have anti-hyperglycemia and anti-diabetic complication effects. The present study therefore investigated the protective mechanism of chebulic acid, a phenolcarboxylic acid compound isolated from the ripe fruits of Terminalia chebula against advanced glycation endproducts (AGEs)-induced endothelial cell dysfunction. To investigate the protective mechanism of chebulic acid against vascular endothelial dysfunction human umbilical vein endothelial cells (HUVEC) were treated with chebulic acid in the presence/absence of glyceraldehyde-related AGEs (glycer-AGEs). HUVEC incubated with 100 μg/ml of glycer-AGEs had significantly enhanced reactive oxygen species formation, whereas the treatment of chebulic acid dose-dependently reduced glycer-AGE-induced formation to 108.2 ± 1.9% for 25 μM versus 137.8 ± 1.1% for glycer-AGEs treated alone. The transendothelial electrical resistance (TER) value of the glycer-AGEs group was dramatically decreased to 76.9 ± 2.2% compared to the control, whereas chebulic acid treatment prevented glycer-AGE-induced TER change with a value of 91.3 ± 5.3%. The incubation of confluent HUVEC with 100 μg/ml of glycer-AGEs for 24 h remarkably increased the adhesion of human monocytic THP-1 cells compared to non-stimulated HUVEC. These increases in HUVEC adhesiveness were dose-dependently reduced by chebulic acid. The present study shows the effects of chebulic acid against the progression of AGE-induced endothelial cell dysfunction suggesting that this compound may constitute a promising intervention agent against diabetic vascular complications.
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2010.07.039